2003
Cleavage of double stranded plasmid DNA by lanthanide complexes
RITTICH, Bohuslav, Alena ŠPANOVÁ, Martin FALK, Milan BENEŠ, Martin HRUBÝ et. al.Basic information
Original name
Cleavage of double stranded plasmid DNA by lanthanide complexes
Authors
RITTICH, Bohuslav (203 Czech Republic, guarantor), Alena ŠPANOVÁ (203 Czech Republic), Martin FALK (203 Czech Republic), Milan BENEŠ (203 Czech Republic) and Martin HRUBÝ (203 Czech Republic)
Edition
Moskva, 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography, p. 126-126, 2003
Publisher
Russian Academy of Sciences
Other information
Language
English
Type of outcome
Proceedings paper
Field of Study
Genetics and molecular biology
Country of publisher
Russian Federation
Confidentiality degree
is not subject to a state or trade secret
RIV identification code
RIV/00216224:14310/03:00008120
Organization unit
Faculty of Science
UT WoS
000187954500023
Keywords in English
plasmid DNA pBR322; cleavage; lanthanide ions; EDTA and IDA complexes; particles
Tags
Changed: 15/7/2003 12:43, doc. Ing. Bohuslav Rittich, CSc.
Abstract
V originále
The use of free lanthanide ions and their complexes for plasmid DNA pBR322 and chromosomal DNA was studied. Plasmid pBR322 DNA was treated by lanthanide clorides in HEPES buffer at different temperatures. Heterogenous lanthanide complexes of EDTA immobilized on insoluble methacrylate support and IDA immobilized on styrene support were used as catalysts for plasmid DNA pBR322 cleavage, too.
Links
| GA202/02/1361, research and development project |
|