Detailed Information on Publication Record
2003
Cytogenetics and cytology of retinoblastomas
BÁRTOVÁ, Eva, Stanislav KOZUBEK, Hana GAJOVÁ, Pavla JIRSOVÁ, Jitka ŽLŮVOVÁ et. al.Basic information
Original name
Cytogenetics and cytology of retinoblastomas
Authors
BÁRTOVÁ, Eva (203 Czech Republic, guarantor), Stanislav KOZUBEK (203 Czech Republic), Hana GAJOVÁ (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Jitka ŽLŮVOVÁ (203 Czech Republic), Renata TASLEROVÁ (203 Czech Republic), Irena KOUTNÁ (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic)
Edition
J Cancer Res. Clin.Oncol. Berlin, Springer-Verlag, 2003, 0171-5216
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.162
RIV identification code
RIV/00216224:14330/03:00008142
Organization unit
Faculty of Informatics
UT WoS
000182651800004
Keywords in English
retinoblastoma; Numerical aberrations; Nuclear topography; Apoptosis
Tags
International impact, Reviewed
Změněno: 7/5/2010 15:52, prof. RNDr. Michal Kozubek, Ph.D.
Abstract
V originále
Using the repeated FISH technique, the average distances between the nuclear membrane and the fluorescence gravity centre (FGC) of seven selected chromosomes were determined in the same tumour population and three other cell types. Chromosome order in positioning from the nuclear membrane was similar in all cell populations investigated. Our experimental studies were focused on specific genetic loci relevant for retinoblastoma tumour pathogenesis. We revealed a certain heterogeneity in the copy number of the Rb1, N-myc and TP53 gene loci in tumour cells. In addition, in lymphocytes isolated from peripheral blood of the patients, a high degree of copy number heterogeneity was also detected. In 60 % of analysed retinoblastomas we observed numerical aberration involving the centromeric region of chromosome 6. In these tumours, apoptotic bodies were found irrespective of clinical therapy. Chromosome instability seems to be a typical feature of primary retinoblastomas as well as of the human pseudodiploid cell line Y79. These cells of a hereditary form of retinoblastoma (Y79) were irradiated by gamma rays and exposed to anti-tumour drugs such as etoposide, vincristine and cisplatin. These treatments induced apoptosis, changes in the cell cycle profile and specific modifications in the nuclear topography of selected loci. Treatment with a non-lethal concentration of hydroxyurea was shown to induce the loss of the amplified N-myc gene involved in the homogenously staining region (HSR) that was found to be associated with the nuclear membrane of retinoblastoma Y79 cells.
Links
| GA301/01/0186, research and development project |
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| IAA5004306, research and development project |
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| IAB5004102, research and development project |
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| IBS5004010, research and development project |
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| MSM 143300002, plan (intention) |
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| NC6987, research and development project |
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