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@article{488213,
author = {Bártová, Eva and Jirsová, Pavla and Fojtová, Miloslava and Souček, Karel and Kozubek, Stanislav},
article_location = {Bäsel},
article_number = {5},
keywords = {Chromosomal territory; Nuclear architecture; Apoptosis; DNA fragmentation},
language = {eng},
issn = {1420-682X},
journal = {Cellular and Molecular Life Sciences},
title = {Chromosomal territory segmentation in apoptotic cells},
volume = {60},
year = {2003}
}
TY - JOUR
ID - 488213
AU - Bártová, Eva - Jirsová, Pavla - Fojtová, Miloslava - Souček, Karel - Kozubek, Stanislav
PY - 2003
TI - Chromosomal territory segmentation in apoptotic cells
JF - Cellular and Molecular Life Sciences
VL - 60
IS - 5
SP - 979-990
EP - 979-990
PB - Birkhaeuser Verlag
SN - 1420682X
KW - Chromosomal territory
KW - Nuclear architecture
KW - Apoptosis
KW - DNA fragmentation
N2 - The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL-60 cells. In K-562 population, chromosomal territories were located separately either in one apoptotic body or to undergo disassembly into chromosomal segments dispersed into single and/or several apoptotic bodies. The apoptotic disorganisation of chromosomal territories was irregular, leading mainly to chromosomal segments of different sizes and, consequently, chromosomal disassembly was not observed at specific sites. Enhanced segmentation was also detected at selected centromeric regions. Sequential staining of the same apoptotic nuclei by the FISH and TUNEL techniques revealed that chromosomal territory segmentation precedes the formation of nuclear apoptotic bodies.
ER -
BÁRTOVÁ, Eva, Pavla JIRSOVÁ, Miloslava FOJTOVÁ, Karel SOUČEK and Stanislav KOZUBEK. Chromosomal territory segmentation in apoptotic cells. \textit{Cellular and Molecular Life Sciences}. Bäsel: Birkhaeuser Verlag, 2003, vol.~60, No~5, p.~979-990. ISSN~1420-682X.
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