| RITTICH, Bohuslav, Alena ŠPANOVÁ, Magdalena SKALNÍKOVÁ and Milan BENEŠ. Chromatographic behaviour and purification of linear lambda phage and plasmid DNA molecules on 2-hydroxyethyl methacrylate-ethylene dimethacrylate-based supports. Journal of Chromatography A. Amsterdam: Elsevier, 2003, vol. 1009, 1-2, p. 207-214. ISSN 0021-9673. |
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@article{488218,
author = {Rittich, Bohuslav and Španová, Alena and Skalníková, Magdalena and Beneš, Milan},
article_location = {Amsterdam},
article_number = {1-2},
keywords = {Stationary phases; LC; slalom chromatography; DNA; hydroxyethyl methacrylate-ethylene dimethacrylate},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Chromatographic behaviour and purification of linear lambda phage and plasmid DNA molecules on 2-hydroxyethyl methacrylate-ethylene dimethacrylate-based supports},
volume = {1009},
year = {2003}
}
TY - JOUR
ID - 488218
AU - Rittich, Bohuslav - Španová, Alena - Skalníková, Magdalena - Beneš, Milan
PY - 2003
TI - Chromatographic behaviour and purification of linear lambda phage and plasmid DNA molecules on 2-hydroxyethyl methacrylate-ethylene dimethacrylate-based supports
JF - Journal of Chromatography A
VL - 1009
IS - 1-2
SP - 207-214
EP - 207-214
PB - Elsevier
SN - 00219673
KW - Stationary phases
KW - LC
KW - slalom chromatography
KW - DNA
KW - hydroxyethyl methacrylate-ethylene dimethacrylate
N2 - The HEMA-BIO 1000 supports based on a copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate were used for separation of lambda DNA and its fragments and plasmid pBR322 DNA. The separation of fragments greater than 6.6 kbp was demonstrated according to the slalom chromatography mechanism on column for gel permeation chromatography in the case of linear lambda DNA fragments. The influence of the particle size of column packing, mobile phase rate, and KCl concentration in mobile phase was discussed. The purification of plasmid DNA pBR322 using GPC was more rapid in comparison with gel electrophoresis. The presence of salts in the eluate is not disadvantageous. DNA can be recovered from the eluate by ethanol precipitation. Plasmid DNA pBR322 isolated in this way was suitable for different biological applications (cleavage with restrictases, electrotransformation into bacterial cells).
ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Magdalena SKALNÍKOVÁ and Milan BENEŠ. Chromatographic behaviour and purification of linear lambda phage and plasmid DNA molecules on 2-hydroxyethyl methacrylate-ethylene dimethacrylate-based supports. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2003, vol.~1009, 1-2, p.~207-214. ISSN~0021-9673.
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