Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

SKLÁDAL, Petr. Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions. Journal of the Brazilian Chemical Society. Sao Paulo: Sociedade Brasileira de Química, 2003, vol. 14, No 4, p. 491-502. ISSN 0103-5053.

Basic information

• Original name: Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
• Authors: SKLÁDAL, Petr (203 Czech Republic, guarantor).
• Edition: Journal of the Brazilian Chemical Society, Sao Paulo, Sociedade Brasileira de Química, 2003, 0103-5053.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Brazil
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.895
• RIV identification code: RIV/00216224:14310/03:00008863
• Organization unit: Faculty of Science
• UT WoS: 000184902600002
• Keywords in English: Piezoelectric biosensor; Review
• Tags: Piezoelectric biosensor, Review

Abstract

This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.

Links

• OC 518.30, research and development project: Name: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministry of Education, Youth and Sports of the CR, Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors