J 2003

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

SKLÁDAL, Petr

Basic information

Original name

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Authors

SKLÁDAL, Petr (203 Czech Republic, guarantor)

Edition

Journal of the Brazilian Chemical Society, Sao Paulo, Sociedade Brasileira de Química, 2003, 0103-5053

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Brazil

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.895

RIV identification code

RIV/00216224:14310/03:00008863

Organization unit

Faculty of Science

UT WoS

000184902600002

Keywords in English

Piezoelectric biosensor; Review

Tags

Změněno: 5/9/2003 14:31, prof. RNDr. Petr Skládal, CSc.

Abstract

V originále

This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.

Links

OC 518.30, research and development project
Name: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů
Investor: Ministry of Education, Youth and Sports of the CR, Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors