New ELISA technique for analysis of p53 protein/DNA binding
properties

J 2002

New ELISA technique for analysis of p53 protein/DNA binding properties

JAGELSKA, E, V. BRAZDA, S POSPISILOVA, B. VOJTESEK, E. PALECEK et. al.

Basic information

Original name

New ELISA technique for analysis of p53 protein/DNA binding properties

Authors

JAGELSKA, E, V. BRAZDA, S POSPISILOVA, B. VOJTESEK and E. PALECEK

Edition

Journal of Immunological Methods, London, Elsevier Science, 2002, 0022-1759

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.598

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/S0022-1759(02)00182-5

UT WoS

000177842300013

Keywords in English

EMSA;p53;DNA-binding

Abstract

V originále

The p53 tumour suppressor protein is one of the most important topics in cancer research. Its function is associated with the ability to bind DNA in a sequence-specific manner and to operate as a transcription factor. In the present study, we have developed a rapid and reliable method for analysing sequence-specific binding of p53 protein to DNA using a modified enzyme-linked immunosorbent assay (ELISA). In this p53/DNA-ELISA, we use streptavidin-coated microplates, to capture biotinylated oligonucleotides containing p53 consensus sequences (p53CON). This newly developed nonradioactive assay allows the detection of p53/DNA complexes using different monoclonal antibodies recognising p53 and has comparable or higher sensitivity to more complicated radioactive methods. Using this method, we can detect binding of endogenous p53 to p53CON and activation of P53 protein for sequence-specific DNA binding. Variations of the basic protocol have also been developed to perform competition experiments and to study p53 binding to natural binding sequences. This modified DNA-ELISA is applicable for screening p53 binding properties from various sources in a short time, (C) 2002 Elsevier Science B.V. All rights reserved.

Citovat

JAGELSKA, E, V. BRAZDA, S POSPISILOVA, B. VOJTESEK and E. PALECEK. New ELISA technique for analysis of p53 protein/DNA binding properties. Journal of Immunological Methods. London: Elsevier Science, 2002, vol. 267, No 2, p. 227-235. ISSN 0022-1759. Available from: https://dx.doi.org/10.1016/S0022-1759(02)00182-5.

@article{488302,
   author = {Jagelska, E and Brazda, V. and Pospisilova, S and Vojtesek, B. and Palecek, E.},
   article_location = {London},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/S0022-1759(02)00182-5},
   keywords = {EMSA;p53;DNA-binding},
   language = {eng},
   issn = {0022-1759},
   journal = {Journal of Immunological Methods},
   title = {New ELISA technique for analysis of p53 protein/DNA binding properties},
   volume = {267},
   year = {2002}
}

TY  - JOUR
ID  - 488302
AU  - Jagelska, E - Brazda, V. - Pospisilova, S - Vojtesek, B. - Palecek, E.
PY  - 2002
TI  - New ELISA technique for analysis of p53 protein/DNA binding properties
JF  - Journal of Immunological Methods
VL  - 267
IS  - 2
SP  - 227-235
EP  - 227-235
PB  - Elsevier Science
SN  - 00221759
KW  - EMSA;p53;DNA-binding
N2  - The p53 tumour suppressor protein is one of the most important topics in cancer research. Its function is associated with the ability to bind DNA in a sequence-specific manner and to operate as a transcription factor. In the present study, we have developed a rapid and reliable method for analysing sequence-specific binding of p53 protein to DNA using a modified enzyme-linked immunosorbent assay (ELISA). In this p53/DNA-ELISA, we use streptavidin-coated microplates, to capture biotinylated oligonucleotides containing p53 consensus sequences (p53CON). This newly developed nonradioactive assay allows the detection of p53/DNA complexes using different monoclonal antibodies recognising p53 and has comparable or higher sensitivity to more complicated radioactive methods. Using this method, we can detect binding of endogenous p53 to p53CON and activation of P53 protein for sequence-specific DNA binding. Variations of the basic protocol have also been developed to perform competition experiments and to study p53 binding to natural binding sequences. This modified DNA-ELISA is applicable for screening p53 binding properties from various sources in a short time, (C) 2002 Elsevier Science B.V. All rights reserved.
ER  -

JAGELSKA, E, V. BRAZDA, S POSPISILOVA, B. VOJTESEK and E. PALECEK. New ELISA technique for analysis of p53 protein/DNA binding properties. \textit{Journal of Immunological Methods}. London: Elsevier Science, 2002, vol.~267, No~2, p.~227-235. ISSN~0022-1759. Available from: https://dx.doi.org/10.1016/S0022-1759(02)00182-5.

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