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@inproceedings{488305,
author = {Wimmerová, Michaela and Imberty, Anne and Sudakevitz, Dvora and Chambat, Gerard and LortatandJacob, Hugues and GilboaandGarber, Nechama},
address = {Bangalore, Indie},
booktitle = {XVII International Symposium on Glycoconjugates},
keywords = {Ralstonia solanacearum; lectin; surface plasmon resonance},
language = {eng},
location = {Bangalore, Indie},
pages = {23-23},
publisher = {AS},
title = {Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.},
year = {2003}
}
TY - JOUR
ID - 488305
AU - Wimmerová, Michaela - Imberty, Anne - Sudakevitz, Dvora - Chambat, Gerard - Lortat-Jacob, Hugues - Gilboa-Garber, Nechama
PY - 2003
TI - Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.
PB - AS
CY - Bangalore, Indie
KW - Ralstonia solanacearum
KW - lectin
KW - surface plasmon resonance
N2 - Ralstonia solanacearum is a worldwide distributed plant aggressive pathogen which causes lethal wilt in many crops. Its extracts contain a fucose-binding lectin that has been recently purified and characterized [1]. Its 90 amino acid sequence contains two repeating domains, with strong similarity to the fucose-binding lectin of the mushroom Aleuria aurantia (AAL), which is also a soil inhabitant. Surface plasmon resonance experiments demonstrate that the lectin binds strongly to fragments of fucose-containing xyloglucan polysaccharide purified from plant cell walls. This binding can diversely be inhibited by fucose and fucose-containing oligosaccharides. Best inhibition was obtained with oligosaccharides containing an aFuc(1-2)Gal terminal disaccharide, particularly XG9 (Glc4 Xyl3 Gal Fuc) which is a structurally well determined plant oligosaccharide that has previously been demonstrated to have biological activity in plants [2]. Our results are in agreement with the assumption that RSL plays a role in binding of the bacterium to specific oligosaccharides that are present in the primary root hairs cell wall of the host plant.
ER -
WIMMEROVÁ, Michaela, Anne IMBERTY, Dvora SUDAKEVITZ, Gerard CHAMBAT, Hugues LORTAT-JACOB a Nechama GILBOA-GARBER. Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum. In \textit{XVII International Symposium on Glycoconjugates}. Bangalore, Indie: AS, 2003, s.~23.
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