Specificity and kinetics of oligosaccharide recognition by RSL,
a fucose-binding lectin from the plant pathogen Ralstonia
solanacearum.

D 2003

Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.

WIMMEROVÁ, Michaela, Anne IMBERTY, Dvora SUDAKEVITZ, Gerard CHAMBAT, Hugues LORTAT-JACOB et. al.

Basic information

Original name

Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.

Authors

WIMMEROVÁ, Michaela (203 Czech Republic, guarantor), Anne IMBERTY (250 France), Dvora SUDAKEVITZ (376 Israel), Gerard CHAMBAT (250 France), Hugues LORTAT-JACOB (250 France) and Nechama GILBOA-GARBER (376 Israel)

Edition

Bangalore, Indie, XVII International Symposium on Glycoconjugates, p. 23-23, 2003

Publisher

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

India

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/03:00008866

Organization unit

Faculty of Science

Keywords in English

Ralstonia solanacearum; lectin; surface plasmon resonance

Abstract

V originále

Ralstonia solanacearum is a worldwide distributed plant aggressive pathogen which causes lethal wilt in many crops. Its extracts contain a fucose-binding lectin that has been recently purified and characterized [1]. Its 90 amino acid sequence contains two repeating domains, with strong similarity to the fucose-binding lectin of the mushroom Aleuria aurantia (AAL), which is also a soil inhabitant. Surface plasmon resonance experiments demonstrate that the lectin binds strongly to fragments of fucose-containing xyloglucan polysaccharide purified from plant cell walls. This binding can diversely be inhibited by fucose and fucose-containing oligosaccharides. Best inhibition was obtained with oligosaccharides containing an aFuc(1-2)Gal terminal disaccharide, particularly XG9 (Glc4 Xyl3 Gal Fuc) which is a structurally well determined plant oligosaccharide that has previously been demonstrated to have biological activity in plants [2]. Our results are in agreement with the assumption that RSL plays a role in binding of the bacterium to specific oligosaccharides that are present in the primary root hairs cell wall of the host plant.

Links

LN00A016, research and development project

Name: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center

Citovat

WIMMEROVÁ, Michaela, Anne IMBERTY, Dvora SUDAKEVITZ, Gerard CHAMBAT, Hugues LORTAT-JACOB and Nechama GILBOA-GARBER. Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum. In XVII International Symposium on Glycoconjugates. Bangalore, Indie: AS, 2003, p. 23.

@inproceedings{488305,
   author = {Wimmerová, Michaela and Imberty, Anne and Sudakevitz, Dvora and Chambat, Gerard and LortatandJacob, Hugues and GilboaandGarber, Nechama},
   address = {Bangalore, Indie},
   booktitle = {XVII International Symposium on Glycoconjugates},
   keywords = {Ralstonia solanacearum; lectin; surface plasmon resonance},
   language = {eng},
   location = {Bangalore, Indie},
   pages = {23-23},
   publisher = {AS},
   title = {Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.},
   year = {2003}
}

TY  - JOUR
ID  - 488305
AU  - Wimmerová, Michaela - Imberty, Anne - Sudakevitz, Dvora - Chambat, Gerard - Lortat-Jacob, Hugues - Gilboa-Garber, Nechama
PY  - 2003
TI  - Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.
PB  - AS
CY  - Bangalore, Indie
KW  - Ralstonia solanacearum
KW  - lectin
KW  - surface plasmon resonance
N2  - Ralstonia solanacearum is a worldwide distributed plant aggressive pathogen which causes lethal wilt in many crops. Its extracts contain a fucose-binding lectin that has been recently purified and characterized [1]. Its 90 amino acid sequence contains two repeating domains, with strong similarity to the fucose-binding lectin of the mushroom Aleuria aurantia (AAL), which is also a soil inhabitant. Surface plasmon resonance experiments demonstrate that the lectin binds strongly to fragments of fucose-containing xyloglucan polysaccharide purified from plant cell walls. This binding can diversely be inhibited by fucose and fucose-containing oligosaccharides. Best inhibition was obtained with oligosaccharides containing an aFuc(1-2)Gal terminal disaccharide, particularly XG9 (Glc4 Xyl3 Gal Fuc) which is a structurally well determined plant oligosaccharide that has previously been demonstrated to have biological activity in plants [2]. Our results are in agreement with the assumption that RSL plays a role in binding of the bacterium to specific oligosaccharides that are present in the primary root hairs cell wall of the host plant.
ER  -

WIMMEROVÁ, Michaela, Anne IMBERTY, Dvora SUDAKEVITZ, Gerard CHAMBAT, Hugues LORTAT-JACOB and Nechama GILBOA-GARBER. Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum. In \textit{XVII International Symposium on Glycoconjugates}. Bangalore, Indie: AS, 2003, p.~23.

Detailed Information on Publication Record