MANNEROVÁ, Sylva, Roman PANTŮČEK, Jiří DOŠKAŘ, Pavel ŠVEC, C. SNAUWAERT, M. VANCANNEYT, J. SWINGS and Ivo SEDLÁČEK. Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas. International Journal of Systematic and Evolutionary Microbiology. UK: Society for General Microbiology, 2003, vol. 53, No 5, p. 1647-1654. ISSN 1466-5026.
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Basic information
Original name Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas
Authors MANNEROVÁ, Sylva (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Jiří DOŠKAŘ (203 Czech Republic), Pavel ŠVEC (203 Czech Republic), C. SNAUWAERT (56 Belgium), M. VANCANNEYT (56 Belgium), J. SWINGS (56 Belgium) and Ivo SEDLÁČEK (203 Czech Republic, guarantor).
Edition International Journal of Systematic and Evolutionary Microbiology, UK, Society for General Microbiology, 2003, 1466-5026.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW [Full Text] [Abstract]
Impact factor Impact factor: 3.187
RIV identification code RIV/00216224:14310/03:00008186
Organization unit Faculty of Science
UT WoS 000185551100066
Keywords in English Macrococcus; Macrococcus brunensis sp. nov.; Macrococcus hajekii sp. nov.; Macrococcus lamae sp. nov.; taxonomy; llama
Tags Llama, Macrococcus, Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov., Macrococcus lamae sp. nov., taxonomy
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Roman Pantůček, Ph.D., učo 842. Changed: 1/7/2009 18:38.
Abstract
Eight strains of Gram-positive, catalase and oxidase positive cocci were isolated from the skin of llamas (Lama glama, L.) and characterized using a polyphasic approach. These strains were assigned to the genus Macrococcus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and the DNA base content of the DNA (40 - 42 mol % G + C). Phylogenetic analysis based on 16S rDNA confirmed that the strains are members of the genus Macrococcus. They differed from all hitherto described macrococcal species in their production of phosphatase, reduction of nitrate (most strains), their inability to produce acid from glycerol and to grow in 7.5 % NaCl. Ribotyping (EcoRI), macrorestriction analysis (XbaI) and fatty acid methyl ester analysis divided all strains from llamas into three stable clusters. Moreover, ribotyping differentiated the strains analyzed not only from previously described macrococcal species but from oxidase positive staphylococci as well. DNA-DNA hybridization confirmed that the three clusters represent separate genomic groups (similarity values were lower than 54 %). All the results showed that the strains represent three new species for which the names Macrococcus hajekii sp. nov. (type strain CCM 4809T = LMG 21711T), Macrococcus brunensis sp. nov. (type strain CCM 4811T = LMG 21712T) and Macrococcus lamae sp. nov. (type strain CCM 4815T = LMG 21713T) are proposed.
Links
GA301/02/1505, research and development projectName: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků
Investor: Czech Science Foundation, Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci
GP204/02/D099, research and development projectName: Knihovna ribotypizačních profilů typových kultur bakterií
Investor: Czech Science Foundation, Database of the ribotype profiles of bacterial type strains
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
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