Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov.
and Macrococcus lamae sp. nov., from the skin of llamas

J 2003

Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas

MANNEROVÁ, Sylva, Roman PANTŮČEK, Jiří DOŠKAŘ, Pavel ŠVEC, C. SNAUWAERT et. al.

Basic information

Original name

Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas

Authors

MANNEROVÁ, Sylva (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Jiří DOŠKAŘ (203 Czech Republic), Pavel ŠVEC (203 Czech Republic), C. SNAUWAERT (56 Belgium), M. VANCANNEYT (56 Belgium), J. SWINGS (56 Belgium) and Ivo SEDLÁČEK (203 Czech Republic, guarantor)

Edition

International Journal of Systematic and Evolutionary Microbiology, UK, Society for General Microbiology, 2003, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

[Full Text] [Abstract]

Impact factor

Impact factor: 3.187

RIV identification code

RIV/00216224:14310/03:00008186

Organization unit

Faculty of Science

UT WoS

000185551100066

Keywords in English

Macrococcus; Macrococcus brunensis sp. nov.; Macrococcus hajekii sp. nov.; Macrococcus lamae sp. nov.; taxonomy; llama

Abstract

V originále

Eight strains of Gram-positive, catalase and oxidase positive cocci were isolated from the skin of llamas (Lama glama, L.) and characterized using a polyphasic approach. These strains were assigned to the genus Macrococcus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and the DNA base content of the DNA (40 - 42 mol % G + C). Phylogenetic analysis based on 16S rDNA confirmed that the strains are members of the genus Macrococcus. They differed from all hitherto described macrococcal species in their production of phosphatase, reduction of nitrate (most strains), their inability to produce acid from glycerol and to grow in 7.5 % NaCl. Ribotyping (EcoRI), macrorestriction analysis (XbaI) and fatty acid methyl ester analysis divided all strains from llamas into three stable clusters. Moreover, ribotyping differentiated the strains analyzed not only from previously described macrococcal species but from oxidase positive staphylococci as well. DNA-DNA hybridization confirmed that the three clusters represent separate genomic groups (similarity values were lower than 54 %). All the results showed that the strains represent three new species for which the names Macrococcus hajekii sp. nov. (type strain CCM 4809T = LMG 21711T), Macrococcus brunensis sp. nov. (type strain CCM 4811T = LMG 21712T) and Macrococcus lamae sp. nov. (type strain CCM 4815T = LMG 21713T) are proposed.

Links

GA301/02/1505, research and development project

Name: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Czech Science Foundation, Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci

GP204/02/D099, research and development project

Name: Knihovna ribotypizačních profilů typových kultur bakterií

Investor: Czech Science Foundation, Database of the ribotype profiles of bacterial type strains

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

MANNEROVÁ, Sylva, Roman PANTŮČEK, Jiří DOŠKAŘ, Pavel ŠVEC, C. SNAUWAERT, M. VANCANNEYT, J. SWINGS and Ivo SEDLÁČEK. Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas. International Journal of Systematic and Evolutionary Microbiology. UK: Society for General Microbiology, 2003, vol. 53, No 5, p. 1647-1654. ISSN 1466-5026.

@article{488746,
   author = {Mannerová, Sylva and Pantůček, Roman and Doškař, Jiří and Švec, Pavel and Snauwaert, C. and Vancanneyt, M. and Swings, J. and Sedláček, Ivo},
   article_location = {UK},
   article_number = {5},
   keywords = {Macrococcus; Macrococcus brunensis sp. nov.; Macrococcus hajekii sp. nov.; Macrococcus lamae sp. nov.; taxonomy; llama},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas},
   url = {http://ijs.sgmjournals.org/cgi/reprint/53/5/1647},
   volume = {53},
   year = {2003}
}

TY  - JOUR
ID  - 488746
AU  - Mannerová, Sylva - Pantůček, Roman - Doškař, Jiří - Švec, Pavel - Snauwaert, C. - Vancanneyt, M. - Swings, J. - Sedláček, Ivo
PY  - 2003
TI  - Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 53
IS  - 5
SP  - 1647-1654
EP  - 1647-1654
PB  - Society for General Microbiology
SN  - 14665026
KW  - Macrococcus
KW  - Macrococcus brunensis sp. nov.
KW  - Macrococcus hajekii sp. nov.
KW  - Macrococcus lamae sp. nov.
KW  - taxonomy
KW  - llama
UR  - http://ijs.sgmjournals.org/cgi/reprint/53/5/1647
N2  - Eight strains of Gram-positive, catalase and oxidase positive cocci were isolated from the skin of llamas (Lama glama, L.) and characterized using a polyphasic approach. These strains were assigned to the genus Macrococcus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and the DNA base content of the DNA (40 - 42 mol % G + C). Phylogenetic analysis based on 16S rDNA confirmed that the strains are members of the genus Macrococcus. They differed from all hitherto described macrococcal species in their production of phosphatase, reduction of nitrate (most strains), their inability to produce acid from glycerol and to grow in 7.5 % NaCl. Ribotyping (EcoRI), macrorestriction analysis (XbaI) and fatty acid methyl ester analysis divided all strains from llamas into three stable clusters. Moreover, ribotyping differentiated the strains analyzed not only from previously described macrococcal species but from oxidase positive staphylococci as well. DNA-DNA hybridization confirmed that the three clusters represent separate genomic groups (similarity values were lower than 54 %). All the results showed that the strains represent three new species for which the names Macrococcus hajekii sp. nov. (type strain CCM 4809T = LMG 21711T), Macrococcus brunensis sp. nov. (type strain CCM 4811T = LMG 21712T) and Macrococcus lamae sp. nov. (type strain CCM 4815T = LMG 21713T) are proposed.
ER  -

MANNEROVÁ, Sylva, Roman PANTŮČEK, Jiří DOŠKAŘ, Pavel ŠVEC, C. SNAUWAERT, M. VANCANNEYT, J. SWINGS and Ivo SEDLÁČEK. $<$I$>$Macrococcus brunensis$<$/I$>$ sp. nov., $<$I$>$Macrococcus hajekii$<$/I$>$ sp. nov. and $<$I$>$Macrococcus lamae$<$/I$>$ sp. nov., from the skin of llamas. \textit{International Journal of Systematic and Evolutionary Microbiology}. UK: Society for General Microbiology, 2003, vol.~53, No~5, p.~1647-1654. ISSN~1466-5026.

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