2003
Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica
STROUHAL, Martin, René KIZEK, Libuše TRNKOVÁ a Miroslav NEMECZákladní údaje
Originální název
Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica
Autoři
STROUHAL, Martin (203 Česká republika), René KIZEK (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, garant) a Miroslav NEMEC (203 Česká republika)
Vydání
Bioelectrochemistry, The Netherlands, Elsevier, 2003, 1567-5394
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10405 Electrochemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.482
Kód RIV
RIV/00216224:14310/03:00008229
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000184909300004
Klíčová slova anglicky
Yeast Yarrowia lipolytica; Differential Pulse Voltammetry; Adsorptive Transfer Stripping Technique; Constant Current Chronopotentiometric Stripping Analysis. Metallothionein; Cadmium; nickel; cobalt and zinc; Bioaccumulation
Štítky
Změněno: 17. 10. 2003 14:46, prof. RNDr. Libuše Trnková, CSc.
Anotace
V originále
The bioaccumulation of heavy metals (cadmium, nickel, cobalt and zinc) and the effect of these metals on the production of metallothionein and metallothionein-like proteins (MT) in Yarrowia lipolytica was studied by electrochemical methods. The concentrations of heavy metals were determined by the differential pulse voltammetry (DPV). A combination of the constant current chronopotentiometric stripping analysis (CPSA) and adsorptive transfer stripping technique (AdTS) was used to determine the content of MT in cells. Both the bioaccumulation of heavy metals and the production of MT in different cell compartments of Yarrowia lipolytica exposed to heavy metals were monitored. The LD50 of each metal was determined from the number of viable cells in yeast cultures. The highest concentrations of heavy metals were found in the cell wall and membrane debris while the lowest concentrations were detected in cytoplasm. Cadmium and nickel showed the most significant effect on the production of MT. This study provides new insights into the ecophysiology of microorganisms and demonstrates the potential use of these electrochemical methods in the biotechnology.
Návaznosti
GA203/02/0422, projekt VaV |
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IAA1163201, projekt VaV |
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