Electrochemical Study of Heavy Metals and Metallothionein in
Yeast Yarrowia lipolytica

J 2003

Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica

STROUHAL, Martin, René KIZEK, Libuše TRNKOVÁ and Miroslav NEMEC

Basic information

Original name

Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica

Authors

STROUHAL, Martin (203 Czech Republic), René KIZEK (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and Miroslav NEMEC (203 Czech Republic)

Edition

Bioelectrochemistry, The Netherlands, Elsevier, 2003, 1567-5394

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.482

RIV identification code

RIV/00216224:14310/03:00008229

Organization unit

Faculty of Science

UT WoS

000184909300004

Keywords in English

Yeast Yarrowia lipolytica; Differential Pulse Voltammetry; Adsorptive Transfer Stripping Technique; Constant Current Chronopotentiometric Stripping Analysis. Metallothionein; Cadmium; nickel; cobalt and zinc; Bioaccumulation

Abstract

V originále

The bioaccumulation of heavy metals (cadmium, nickel, cobalt and zinc) and the effect of these metals on the production of metallothionein and metallothionein-like proteins (MT) in Yarrowia lipolytica was studied by electrochemical methods. The concentrations of heavy metals were determined by the differential pulse voltammetry (DPV). A combination of the constant current chronopotentiometric stripping analysis (CPSA) and adsorptive transfer stripping technique (AdTS) was used to determine the content of MT in cells. Both the bioaccumulation of heavy metals and the production of MT in different cell compartments of Yarrowia lipolytica exposed to heavy metals were monitored. The LD50 of each metal was determined from the number of viable cells in yeast cultures. The highest concentrations of heavy metals were found in the cell wall and membrane debris while the lowest concentrations were detected in cytoplasm. Cadmium and nickel showed the most significant effect on the production of MT. This study provides new insights into the ecophysiology of microorganisms and demonstrates the potential use of these electrochemical methods in the biotechnology.

Links

GA203/02/0422, research and development project

Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry

IAA1163201, research and development project

Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.

Citovat

STROUHAL, Martin, René KIZEK, Libuše TRNKOVÁ and Miroslav NEMEC. Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica. Bioelectrochemistry. The Netherlands: Elsevier, 2003, neuveden, No 60, p. 29-36. ISSN 1567-5394.

@article{489502,
   author = {Strouhal, Martin and Kizek, René and Trnková, Libuše and Nemec, Miroslav},
   article_location = {The Netherlands},
   article_number = {60},
   keywords = {Yeast Yarrowia lipolytica; Differential Pulse Voltammetry; Adsorptive Transfer Stripping Technique; Constant Current Chronopotentiometric Stripping Analysis. Metallothionein; Cadmium; nickel; cobalt and zinc; Bioaccumulation},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica},
   volume = {neuveden},
   year = {2003}
}

TY  - JOUR
ID  - 489502
AU  - Strouhal, Martin - Kizek, René - Trnková, Libuše - Nemec, Miroslav
PY  - 2003
TI  - Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica
JF  - Bioelectrochemistry
VL  - neuveden
IS  - 60
SP  - 29-36
EP  - 29-36
PB  - Elsevier
SN  - 15675394
KW  - Yeast Yarrowia lipolytica
KW  - Differential Pulse Voltammetry
KW  - Adsorptive Transfer Stripping Technique
KW  - Constant Current Chronopotentiometric Stripping Analysis. Metallothionein
KW  - Cadmium
KW  - nickel
KW  - cobalt and zinc
KW  - Bioaccumulation
N2  - The bioaccumulation of heavy metals (cadmium, nickel, cobalt and zinc) and the effect of these metals on the production of metallothionein and metallothionein-like proteins (MT) in Yarrowia lipolytica was studied by electrochemical methods. The concentrations of heavy metals were determined by the differential pulse voltammetry (DPV). A combination of the constant current chronopotentiometric stripping analysis (CPSA) and adsorptive transfer stripping technique (AdTS) was used to determine the content of MT in cells. Both the bioaccumulation of heavy metals and the production of MT in different cell compartments of Yarrowia lipolytica exposed to heavy metals were monitored. The LD50 of each metal was determined from the number of viable cells in yeast cultures. The highest concentrations of heavy metals were found in the cell wall and membrane debris while the lowest concentrations were detected in cytoplasm. Cadmium and nickel showed the most significant effect on the production of MT. This study provides new insights into the ecophysiology of microorganisms and demonstrates the potential use of these electrochemical methods in the biotechnology.
ER  -

STROUHAL, Martin, René KIZEK, Libuše TRNKOVÁ and Miroslav NEMEC. Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2003, neuveden, No~60, p.~29-36. ISSN~1567-5394.

Detailed Information on Publication Record