Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9

TRNKOVÁ, Libuše, František JELEN and Irena POSTBIEGLOVÁ. Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9. Electroanalysis. Weinheim, Germany: WILEY-VCH Verlag GmbH, 2003, vol. 19, No 15, p. 1529-1535. ISSN 1040-0397.

Basic information

• Original name: Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9
• Authors: TRNKOVÁ, Libuše (203 Czech Republic, guarantor), František JELEN (203 Czech Republic) and Irena POSTBIEGLOVÁ (203 Czech Republic).
• Edition: Electroanalysis, Weinheim, Germany, WILEY-VCH Verlag GmbH, 2003, 1040-0397.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10405 Electrochemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.811
• RIV identification code: RIV/00216224:14310/03:00008231
• Organization unit: Faculty of Science
• Keywords in English: Reduction of adenine and cytosine in oligonucleotides; mercury electrode; elimination voltammetry with linear scan; dC9 and dA9
• Tags: dC9 and dA9, Mercury Electrode

Abstract

Elimination voltammetry with linear scan (EVLS) has been applied to the resolution of reduction signals of adenine and cytosine in short synthetic homo-oligodeoxynucleotides (dA9 and dC9). In comparison with the common electrochemical methods (linear sweep, differential pulse, and square wave voltammetry) EVLS enables to resolve the overlapped signals by using the function which eliminates the charging and kinetic currents ( , ) and conserves the diffusion current ( ). For the adsorbed electroactive substance, this elimination function gives a well readable peak-counterpeak which has successfully been utilized to the analysis of overlapped reduction signals of adenine and cytosine on hanging mercury drop electrode (HMDE). The height and potential of signals studied were affected by the dC9/dA9 ratio, the time of accumulation, the stirring speed during the adsorption, and pH. Our results showed that EVLS in connection with the adsorption procedure is a useful tool for qualitative and quantitative studies of short oligonucleotides.

Links

• GA203/02/0422, research and development project: Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry

• GV204/97/K084, research and development project: Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

• IAA1163201, research and development project: Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.