Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9

TRNKOVÁ, Libuše, František JELEN and Irena POSTBIEGLOVÁ. Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9. Electroanalysis. Weinheim, Germany: WILEY-VCH Verlag GmbH, 2003, vol. 19, No 15, p. 1529-1535. ISSN 1040-0397.

Basic information

Original name

Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9

Authors

TRNKOVÁ, Libuše (203 Czech Republic, guarantor), František JELEN (203 Czech Republic) and Irena POSTBIEGLOVÁ (203 Czech Republic)

Edition

Electroanalysis, Weinheim, Germany, WILEY-VCH Verlag GmbH, 2003, 1040-0397

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.811

RIV identification code

RIV/00216224:14310/03:00008231

Organization unit

Faculty of Science

Keywords in English

Reduction of adenine and cytosine in oligonucleotides; mercury electrode; elimination voltammetry with linear scan; dC9 and dA9

Tags

dC9 and dA9, Mercury Electrode

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Abstract

V originále

Elimination voltammetry with linear scan (EVLS) has been applied to the resolution of reduction signals of adenine and cytosine in short synthetic homo-oligodeoxynucleotides (dA9 and dC9). In comparison with the common electrochemical methods (linear sweep, differential pulse, and square wave voltammetry) EVLS enables to resolve the overlapped signals by using the function which eliminates the charging and kinetic currents ( , ) and conserves the diffusion current ( ). For the adsorbed electroactive substance, this elimination function gives a well readable peak-counterpeak which has successfully been utilized to the analysis of overlapped reduction signals of adenine and cytosine on hanging mercury drop electrode (HMDE). The height and potential of signals studied were affected by the dC9/dA9 ratio, the time of accumulation, the stirring speed during the adsorption, and pH. Our results showed that EVLS in connection with the adsorption procedure is a useful tool for qualitative and quantitative studies of short oligonucleotides.

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Links

GA203/02/0422, research and development project	Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
GV204/97/K084, research and development project	Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
IAA1163201, research and development project	Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.