D 2003

Topography of telomeres and structure of chromosome territories in human lymphocytes

AMRICHOVÁ, Jana, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ and Michal KOZUBEK

Basic information

Original name

Topography of telomeres and structure of chromosome territories in human lymphocytes

Authors

AMRICHOVÁ, Jana (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic, guarantor)

Edition

Brno, Biophysics of the Genome, p. 1-11, 11 pp. 2003

Publisher

Masaryk University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/03:00008239

Organization unit

Faculty of Informatics

ISBN

80-210-3226-X

Keywords in English

nuclear architecture; telomere; centromere; chromosome territory; chromatin structure; interphase chromosome; human lymphocytes; telomere association; telomere tethering

Tags

Tags

International impact
Změněno: 29/10/2010 14:38, prof. RNDr. Michal Kozubek, Ph.D.

Abstract

V originále

Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied by repeated FISH, high-resolution cytometry and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the opposite side of the territories as compared with the centromeres for all chromosome territories investigated. Mutual distances between telomeres of submetacentric chromosomes were very short, usually shorter than centromere-to-telomere distances, which means that the chromosome territory is non-randomly folded. Telomeres are in average much nearer to the center of the cell nucleus than centromeres; q-telomeres were found in average more centrally localized as compared with p-telomeres. Consequently, we directly showed that chromosome territories in the cell nucleus are (i) polar and (ii) partially oriented in cell nuclei. The distributions of genetic elements relative to chromosome territories (territorial distributions) can be either narrower or broader than their nuclear distributions, which reflects the degree of adhesion of an element to the territory or to the nucleus.

Links

GA202/02/0804, research and development project
Name: Určení radiačního rizika pro vznik chronické myeloidní leukémie na základě měření vzdáleností mezi geny ABL a BCR v hematopoietických buňkách
Investor: Czech Science Foundation, Chronic myeloid leukemia radiation risk estimation based on BCR-ABL distances in hemotopoietic cells
GA301/01/0186, research and development project
Name: Studium lokální kontroly exprese genů pomocí spektrální mikroskopie a analýzy obrazu
Investor: Czech Science Foundation, Investigation of the local control of gene expression using spectral microscopy and omage analysis techniques
IAA5004306, research and development project
Name: Struktura lidského genomu
Investor: Academy of Sciences of the Czech Republic, Human genome structure
IBS5004010, research and development project
Name: Vývoj nových diagnostických technik pro onkologii
Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
ME 565, research and development project
Name: Struktura a funkce interfázních chromosomů
MSM 143300002, plan (intention)
Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
NC6987, research and development project
Name: Epigeneticky kontrolované změny exprese genů u nádorových onemocnění
Investor: Ministry of Health of the CR, Epigenetic control of gene expression in malignant diseases