Detailed Information on Publication Record
2003
Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize
MASAŘÍK, Michal, René KIZEK, K.J. KRAMER, Sabina BILLOVÁ, Marie BRÁZDOVÁ et. al.Basic information
Original name
Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize
Name in Czech
Aplikace avidin-biotinové technologie a adsorptivní přenosové rozpouštěcí square-wave voltametrie pro detekci DNA hybridizace a avidinu v transgenní avidinové kukuřici
Authors
MASAŘÍK, Michal (203 Czech Republic), René KIZEK (203 Czech Republic, guarantor), K.J. KRAMER (840 United States of America), Sabina BILLOVÁ (203 Czech Republic), Marie BRÁZDOVÁ (203 Czech Republic), Jan VACEK (203 Czech Republic), Michele BAILEY (840 United States of America), František JELEN (203 Czech Republic) and A. J. HOWARD (840 United States of America)
Edition
Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2003, 0003-2700
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 5.250
Organization unit
Faculty of Science
UT WoS
000183397500022
Keywords in English
Streptavidin; Avidin; Adorptive Transfer Stripping Voltammetry; Square-Wave Voltammetry; Biotin Binding; Transgenic Avidin Maize; Corn; Grain; Transgenic Protein Detection; Electrochemical Detection; DNA Hybridization
Tags
Tags
International impact, Reviewed
Změněno: 25/6/2009 10:45, prof. RNDr. Michal Masařík, Ph.D.
V originále
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quatifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
In Czech
Práce popisuje využití avidin-biotinové technologie a square wave voltametrie (SWV) v kombinaci s adsorptivní rozpouštěcí přenosovou technikou (AdTS) pro stanovení samotného avidinu a streptavidinu s použitím uhlíkové pastové elektrody a dále využití modifikované pastové elektrody pro detekci DNA hybridizace a ke stanovení avidinu v transgenní rostlině (Zea mays), do které byl vnesen gen pro avidin.
Links
GA203/02/0422, research and development project |
| ||
IAA1163201, research and development project |
|