Analysis of DNA methylation status of cell nuclei using
high-resolution

D 2003

Analysis of DNA methylation status of cell nuclei using high-resolution

SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Stanislav KOZUBEK, Michal KOZUBEK et. al.

Basic information

Original name

Analysis of DNA methylation status of cell nuclei using high-resolution

Authors

SKALNÍKOVÁ, Magdalena (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Pavla GAJDUŠKOVÁ (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic, guarantor) and Michal KOZUBEK (203 Czech Republic)

Edition

Brno, Biophysics of the Genome, p. 59-61, 3 pp. 2003

Publisher

Masaryk University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/03:00009009

Organization unit

Faculty of Informatics

ISBN

80-210-3226-X

Keywords in English

chromatin structure

Abstract

V originále

Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20. Sequential 5-methylcytosin (5-mC)-rich regions were visualised on metaphase chromosomes 1,10, 18, 19, and 20 using immunofluorescent labelling. The aim of this work was to study the methylation status at the chromosomal level in different cell types.

Links

IAB5004102, research and development project

Name: Jaderná topologie některých protoonkogenů u lidských neutrofilních granulocytů a leukemických buněk

Investor: Academy of Sciences of the Czech Republic, Nuclear topology of some protooncogenes in human neutrophilic granulocytes and leukemic cells

MSM 143300002, plan (intention)

Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

Citovat

SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Stanislav KOZUBEK and Michal KOZUBEK. Analysis of DNA methylation status of cell nuclei using high-resolution. In Biophysics of the Genome. Brno: Masaryk University, 2003, p. 59-61. ISBN 80-210-3226-X.

@inproceedings{489662,
   author = {Skalníková, Magdalena and Bártová, Eva and Gajdušková, Pavla and Kozubek, Stanislav and Kozubek, Michal},
   address = {Brno},
   booktitle = {Biophysics of the Genome},
   keywords = {chromatin structure},
   language = {eng},
   location = {Brno},
   isbn = {80-210-3226-X},
   pages = {59-61},
   publisher = {Masaryk University},
   title = {Analysis of DNA methylation status of cell nuclei using high-resolution},
   year = {2003}
}

TY  - JOUR
ID  - 489662
AU  - Skalníková, Magdalena - Bártová, Eva - Gajdušková, Pavla - Kozubek, Stanislav - Kozubek, Michal
PY  - 2003
TI  - Analysis of DNA methylation status of cell nuclei using high-resolution
PB  - Masaryk University
CY  - Brno
SN  - 802103226X
KW  - chromatin structure
N2  - Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20. Sequential 5-methylcytosin (5-mC)-rich regions were visualised on metaphase chromosomes 1,10, 18, 19, and 20 using immunofluorescent labelling. The aim of this work was to study the methylation status at the chromosomal level in different cell types.
ER  -

SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Stanislav KOZUBEK and Michal KOZUBEK. Analysis of DNA methylation status of cell nuclei using high-resolution. In \textit{Biophysics of the Genome}. Brno: Masaryk University, 2003, p.~59-61. ISBN~80-210-3226-X.

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