The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots

SÝKOROVÁ, Eva, Kar Yoong LIM, Mark W. CHASE, Sandra KNAPP, Ilia Judith LEITCH, Andrew Rowland LEITCH and Jiří FAJKUS. The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots. Plant Journal. Oxford: Blackwell Publishing Ltd, 2003, Neuveden, No 34, p. 283-291. ISSN 0960-7412.

Basic information

• Original name: The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots
• Authors: SÝKOROVÁ, Eva (203 Czech Republic), Kar Yoong LIM (826 United Kingdom of Great Britain and Northern Ireland), Mark W. CHASE (826 United Kingdom of Great Britain and Northern Ireland), Sandra KNAPP (826 United Kingdom of Great Britain and Northern Ireland), Ilia Judith LEITCH (826 United Kingdom of Great Britain and Northern Ireland), Andrew Rowland LEITCH (826 United Kingdom of Great Britain and Northern Ireland) and Jiří FAJKUS (203 Czech Republic, guarantor).
• Edition: Plant Journal, Oxford, Blackwell Publishing Ltd, 2003, 0960-7412.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 5.914
• RIV identification code: RIV/00216224:14310/03:00008258
• Organization unit: Faculty of Science
• UT WoS: 000182455200003
• Keywords in English: absence of TTTAGGG telomere; dicot; chromosome; FISH
• Tags: absence of TTTAGGG telomere, CHROMOSOME, dicot, FISH
• Tags: International impact, Reviewed

Abstract

Using slot-blot and fluorescent in situ hybridization (FISH), we found no evidence for the presence of the Arabidopsis-type telomeric sequence (TTTAGGG)n at the chromosome termini in any of the Cestrum species we investigated. Probing for the human-type telomere (TTAGGG)n also revealed no signal. However, polymerase chain reaction experiments indicated that there are short lengths of the sequence TTTAGGG dispersed in the genome but that these sequences are almost certainly too short to act as functional telomeres even if they were at the chromosome termini. An analysis of related genera Vestia and Sessea indicates that they too lack the Arabidopsis-type telomere, and the sequences were lost in the common ancestor of these genera. We found that the Cestrum species investigated had particularly large mean chromosome sizes. We discuss whether this is a consequence of alternative telomere end maintenance systems.

Links

• GA204/02/0027, research and development project: Name: Molekulární analýza struktury a funkce typických a alternativních telomer u rostlin
• MSM 143100008, plan (intention): Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions