Structural basis of calcium and galactose recognition by the
lectin PA IL of Pseudomonas aeruginosa

J 2003

Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa

CIOCCI, Gianluca, Edward MITCHELL, Catherine GAUIER, Michaela WIMMEROVÁ, Dvora SUDAKEVITZ et. al.

Basic information

Original name

Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa

Authors

CIOCCI, Gianluca (380 Italy), Edward MITCHELL (826 United Kingdom of Great Britain and Northern Ireland), Catherine GAUIER (250 France), Michaela WIMMEROVÁ (203 Czech Republic, guarantor), Dvora SUDAKEVITZ (376 Israel), Serge PÉREZ (250 France), Nechama GILBOA-GARBER (376 Israel) and Anne IMBERTY (250 France)

Edition

FEBS Letters, Amsterdam, Elsevier, 2003, 0014-5793

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.609

RIV identification code

RIV/00216224:14310/03:00009169

Organization unit

Faculty of Science

Keywords in English

pseudomonas aeruginosa; cystic fibrosis; lectin; crystal structure

Abstract

V originále

The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 Å esolution, and the native protein was solved at 2.4 Å resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the coordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent to that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.

Links

ME 675, research and development project

Name: Strukturně- funkční studie lektinů bakteriálních patogenů

MSM 143100005, plan (intention)

Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism

Citovat

CIOCCI, Gianluca, Edward MITCHELL, Catherine GAUIER, Michaela WIMMEROVÁ, Dvora SUDAKEVITZ, Serge PÉREZ, Nechama GILBOA-GARBER and Anne IMBERTY. Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa. FEBS Letters. Amsterdam: Elsevier, 2003, vol. 552, No 2, p. 297-301. ISSN 0014-5793.

@article{490424,
   author = {Ciocci, Gianluca and Mitchell, Edward and Gauier, Catherine and Wimmerová, Michaela and Sudakevitz, Dvora and Pérez, Serge and GilboaandGarber, Nechama and Imberty, Anne},
   article_location = {Amsterdam},
   article_number = {2},
   keywords = {pseudomonas aeruginosa; cystic fibrosis; lectin; crystal structure},
   language = {eng},
   issn = {0014-5793},
   journal = {FEBS Letters},
   title = {Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa},
   volume = {552},
   year = {2003}
}

TY  - JOUR
ID  - 490424
AU  - Ciocci, Gianluca - Mitchell, Edward - Gauier, Catherine - Wimmerová, Michaela - Sudakevitz, Dvora - Pérez, Serge - Gilboa-Garber, Nechama - Imberty, Anne
PY  - 2003
TI  - Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa
JF  - FEBS Letters
VL  - 552
IS  - 2
SP  - 297-301
EP  - 297-301
PB  - Elsevier
SN  - 00145793
KW  - pseudomonas aeruginosa
KW  - cystic fibrosis
KW  - lectin
KW  - crystal structure
N2  - The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 Å esolution, and the native protein was solved at 2.4 Å resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the coordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent to that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
ER  -

CIOCCI, Gianluca, Edward MITCHELL, Catherine GAUIER, Michaela WIMMEROVÁ, Dvora SUDAKEVITZ, Serge PÉREZ, Nechama GILBOA-GARBER and Anne IMBERTY. Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa. \textit{FEBS Letters}. Amsterdam: Elsevier, 2003, vol.~552, No~2, p.~297-301. ISSN~0014-5793.

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