KUCHAŘ, Milan, Mirka HORÁKOVÁ, Dagmar ZACHOVÁ and Jiří FAJKUS. Hledání rostlinných telomer-vazebných proteinů prostřednictvím genomického a proteomického přístupu (Searching for plant telomere-binding proteins using genomic and proteomic approaches). In VII. Pracovní setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita v Brně, 2003, p. 27. ISBN 80-210-3053-4.
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Basic information
Original name Hledání rostlinných telomer-vazebných proteinů prostřednictvím genomického a proteomického přístupu
Authors KUCHAŘ, Milan (203 Czech Republic), Mirka HORÁKOVÁ (203 Czech Republic), Dagmar ZACHOVÁ (203 Czech Republic) and Jiří FAJKUS (203 Czech Republic, guarantor).
Edition Brno, VII. Pracovní setkání biochemiků a molekulárních biologů, p. 27-27, 2003.
Publisher Masarykova univerzita v Brně
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/03:00008350
Organization unit Faculty of Science
ISBN 80-210-3053-4
Keywords in English telomere-binding proteins; Arabidopsis thaliana
Tags Arabidopsis thaliana, telomere-binding proteins
Changed by Changed by: prof. RNDr. Jiří Fajkus, CSc., učo 28574. Changed: 24/6/2008 16:06.
Abstract
Two proteins, AtTBP2 (acc. nos. T46051 (protein database), GI:638639 (nucleotide database)) and AtTBP3 (BAB08466, GI:9757879) from Arabidopsis thaliana, were found in the database on the basis of amino acid sequence similarity with telomere-binding protein PcMyb1 (acc. No. T15038) from Petroselinum crispum. Both of the proteins were expressed in various A. thaliana tissues and as the PcMyb2 contain Myb-like domain at the N-terminus which enables the double-stranded DNA binding and additionally a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. The cDNAs of ATTB1 and ATTB2 were cloned and the experiments carried out in Saccharomyces cerevisiae using the two-hybrid system showed interaction between the two proteins and self-interaction of each of the proteins.
Links
GA204/02/0027, research and development projectName: Molekulární analýza struktury a funkce typických a alternativních telomer u rostlin
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
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