Changes of immunofluorescence staining for ECM molecules in the
satellite cells of rat dorsal root ganglia following a chronic
constriction injury of the peripheral nerve

J 2003

Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve

DUBOVÝ, Petr, Radim JANČÁLEK and Ilona KLUSÁKOVÁ

Basic information

Original name

Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor), Radim JANČÁLEK (203 Czech Republic) and Ilona KLUSÁKOVÁ (203 Czech Republic)

Edition

Glia Suppl, USA, Wiley-Liss, Inc. 2003, 0894-1491

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.677

RIV identification code

RIV/00216224:14110/03:00008386

Organization unit

Faculty of Medicine

UT WoS

000184938300154

Keywords in English

laminin; chondroitin sulfate; tenascin; fibronectin; endoneurium

Abstract

ORIG CZ

V originále

We have investigated immunostaining for some ECM molecules ( chondroitin sulfate, tenascin, thrombospondin, fibronectin) in the DRG satellite cells following CCI of the peripheral nerve. The experimental CCI was applied ipsilaterally on the spinal nerve and the sciatic nerve of Wistar rats (12 females, 280g). Both ipsilateral and contralateral DRG (L4-L5) were removed 1, 2, 3, 5 weeks after the operation. The immunofluorescence staining for ECM molecules was compared in the sections through ipsilateral and contralateral DRG by an image analysis system. The satellite cell sheaths of ipsilateral DRG (L4-L5) displayed a higher brightness of immunofluorescence staining for investigated ECM molecules in comparison to the contralateral ones. The changes were more noticeable after CCI applied near to DRG (4mm) than in longer distance (40mm). An increased immunostaining for the ECM molecules in the satellite cells following CCI of peripheral nerve suggested that the changes may be implicated in growth stimuli for sympathetic sprouts and formation of sympathetic baskets.

In Czech

Molekuly ECM (CSPG, tenascin, thrombospondin, fibronectin) byly imunohistochemicky detekovány ve spinálních gangliích po chronické konstrikci nervu.

Links

GA309/00/0407, research and development project

Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves

Citovat

DUBOVÝ, Petr, Radim JANČÁLEK and Ilona KLUSÁKOVÁ. Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve. Glia Suppl. USA: Wiley-Liss, Inc., 2003, vol. 2, No 1, p. 36-37. ISSN 0894-1491.

@article{490969,
   author = {Dubový, Petr and Jančálek, Radim and Klusáková, Ilona},
   article_location = {USA},
   article_number = {1},
   keywords = {laminin; chondroitin sulfate; tenascin; fibronectin; endoneurium},
   language = {eng},
   issn = {0894-1491},
   journal = {Glia Suppl},
   title = {Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve},
   volume = {2},
   year = {2003}
}

TY  - JOUR
ID  - 490969
AU  - Dubový, Petr - Jančálek, Radim - Klusáková, Ilona
PY  - 2003
TI  - Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve
JF  - Glia Suppl
VL  - 2
IS  - 1
SP  - 36
EP  - 36
PB  - Wiley-Liss, Inc.
SN  - 08941491
KW  - laminin
KW  - chondroitin sulfate
KW  - tenascin
KW  - fibronectin
KW  - endoneurium
N2  - We have investigated immunostaining for some ECM molecules ( chondroitin sulfate, tenascin, thrombospondin, fibronectin) in the DRG satellite cells following CCI of the peripheral nerve. The experimental CCI was applied ipsilaterally on the spinal nerve and the sciatic nerve of Wistar rats (12 females, 280g). Both ipsilateral and contralateral DRG (L4-L5) were removed 1, 2, 3, 5 weeks after the operation. The immunofluorescence staining for ECM molecules was compared in the sections through ipsilateral and contralateral DRG by an image analysis system. The satellite cell sheaths of ipsilateral DRG (L4-L5) displayed a higher brightness of immunofluorescence staining for investigated ECM molecules in comparison to the contralateral ones. The changes were more noticeable after CCI applied near to DRG (4mm) than in longer distance (40mm). An increased immunostaining for the ECM molecules in the satellite cells following CCI of peripheral nerve suggested that the changes may be implicated in growth stimuli for sympathetic sprouts and formation of sympathetic baskets.
ER  -

DUBOVÝ, Petr, Radim JANČÁLEK and Ilona KLUSÁKOVÁ. Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve. \textit{Glia Suppl}. USA: Wiley-Liss, Inc., 2003, vol.~2, No~1, p.~36-37. ISSN~0894-1491.

Detailed Information on Publication Record