J 2003

Review: Advances in Capillary Electrophoretically Mediated Microanalysis

VAN DYCK, Sigrid, Eliangiringa KAALE, Soňa VYTISKOVÁ, Zdeněk GLATZ, Jos HOOGMARTENS et. al.

Basic information

Original name

Review: Advances in Capillary Electrophoretically Mediated Microanalysis

Authors

VAN DYCK, Sigrid (56 Belgium), Eliangiringa KAALE (56 Belgium), Soňa VYTISKOVÁ (203 Czech Republic), Zdeněk GLATZ (203 Czech Republic, guarantor), Jos HOOGMARTENS (56 Belgium) and Ann VAN SCHEPDAEL (56 Belgium)

Edition

Electrophoresis, Weinheim, WILEY-VCH Verlag GmbH, 2003, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.040

RIV identification code

RIV/00216224:14310/03:00008401

Organization unit

Faculty of Science

Keywords in English

Capillary electrophoresis Electrophoretically mediated microanalysis Enzyme Derivatization Review
Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Abstract

V originále

This survey gives an overview of recent developments in the use of capillary electrophoretic techniques for the in-line study of enzyme reactions and derivatization protocols. The article is divided into two parts: one concerning in-line enzyme reactions and one concerning in-capillary derivatization. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. Next, a literature overview is provided, starting from 1996, and the investigated enzymes are classified into two tables based on the mode of engagement (i.e. continuous or transient) of the developed EMMA based assay. The second part starts with an introduction of the procedures and the nomenclature used in the area of in-line derivatization protocols based on EMMA. Reported derivatization procedures are discussed and classified in tables, divided in sections following the functional group that is derivatized. Finally, conclusion.

Links

GA203/03/1125, research and development project
Name: Využití kapilární zónové elektroforézy pro studium enzymů
Investor: Czech Science Foundation, Application of capillary zone electrophoresis for the study of enzymes