Polypodium x mantoniae and new localities of P. interjectum in the Czech Republic confirmed using flow cytometry

BUREŠ, Petr, Yi-Feng WANG, Lubomír TICHÝ and Jan BARTOŠ. Polypodium x mantoniae and new localities of P. interjectum in the Czech Republic confirmed using flow cytometry. Preslia. Praha, 2003, vol. 75, No 4, p. 293-310. ISSN 0032-7786.

Basic information

• Original name: Polypodium x mantoniae and new localities of P. interjectum in the Czech Republic confirmed using flow cytometry
• Name in Czech: Polypodium x mantoniae a nové lokality P. interjectum v České republice, potvrzené pomocí průtokové cytometrie
• Authors: BUREŠ, Petr (203 Czech Republic, guarantor, belonging to the institution), Yi-Feng WANG (156 China), Lubomír TICHÝ (203 Czech Republic, belonging to the institution) and Jan BARTOŠ (203 Czech Republic).
• Edition: Preslia, Praha, 2003, 0032-7786.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/03:00009344
• Organization unit: Faculty of Science
• UT WoS: 000187617600001
• Keywords in English: AT frequence;C-value;ferns;flow cytometry;genome size;nuclear DNA content;PI/DAPI index;Polypodiaceae;Pteridophyta
• Tags: AT frequence, C-value, ferns, flow cytometry, genome size, nuclear DNA content, PI/DAPI index, Polypodiaceae, Polypodiophyta, Pteridophyta
• Tags: International impact, Reviewed

Abstract

Flow cytometry measurements confirmed the occurrence of Polypodium x mantoniae (P. interjectum x P. vulgare) in three localities from the eastern part of the Czech Republic (Blansko and Rudice North of Brno and Javoříčko WNW of Olomouc). Nuclear DNA contents (+/- Sx) were determined for P. vulgare (2C=29.00 +/- 0.32 pg), P. x mantoniae (2C=37.18 +/- 0.38 pg) and P. interjectum (2C=45.24 +/- 0.31 pg) by a PAS Partec GmbH flow cytometer (PI staining / standard Vicia faba, 2C=26.9 pg). The relative DNA content ratio was measured between all pairs of taxa (plus minus Sx range) P. vulgare : P. x mantoniae = 0.746 plus minus 0.005; P. vulgare : P. interjectum = 0.595 plus minus 0.001; P. x mantoniae : P. vulgare = 1.340 plus minus 0.008; P. x mantoniae : P. interjectum = 0.797 plus minus 0.005; P. interjectum : P. vulgare = 1.681 plus minus 0.003; P. interjectum : P. x mantoniae = 1.255 plus minus 0.008. Six new localities of Polypodium interjectum were found in the region of Moravský Kras (= Moravian Karst, north of Brno). From the PI/DAPI index it can be inferred that the AT/GC ratio (or heterochromatin occurrence) is 1.05x bigger in P. x mantoniae than in P. vulgare and 1.08x bigger in P. interjectum than in P. vulgare. Anatomical data (number of thick-walled cells of the anulus, spore length and stomata length) of selected specimens and live samples from the Czech Republic were in good agreement with the range of variation of these features published by earlier authors from other European countries. A brief historical survey of knowledge of P. interjectum in the Czech Republic is included.

Abstract (in Czech)

Cytometricky byl potvrzen výskyt křížence Polypodium xmantoniae (P. interjectum x P. vulgare) na třech lokalitách v České republice: Javoříčko, nad vchodem do Javoříčských jeskyní (L. Tichý 15. 2. 2002 BRNU); Blansko, v Suchém žlebu asi 3 km SSV Skalního Mlýna (L. Tichý 8. 7. 2002 BRNU); Rudice, Rudické propadání (L. Tichý 25. 6. 2002 BRNU). Revizí herbářového materiálu byl dále tento kříženec prokázán z lokalit: Blansko, Bílá skála za Černými v Josefovském údolí (F. Bílý 1935 BRNU) a Blansko, u mostu Černých v Josefovském údolí (F. Bílý 1935 BRNU). Cytometricky byl dále doložen z více lokalit také P. interjectum: – Adamov, u jeskyně Kostelík v Josefovském údolí (L. Tichý 21. 5. 2002 BRNU); Adamov, u jeskyně Jáchymka v Josefovském údolí (L. Tichý 12. 6. 2002 BRNU); Adamov, u jeskyně Býčí skála v Josefovském údolí (L. Tichý 21. 5. 2002 BRNU); Blansko, skály na levé straně silnice u Čertova mostu v Suchém žlebu ca 1 km SSV od Kateřinských jeskyní (L. Tichý 8. 7. 2002 BRNU); Babice nad Svitavou, skalnatý svah 0,5 km JV od obce v chráněném území Čihadlo (L. Tichý 12. 6. 2002 BRNU); Ochoz u Brna, údolí Říčky ca 0,3 km Z od vodní nádrže Hádek (L. Tichý 25. 6. 2002 BRNU). Absolutní obsah jaderné DNA (+/- Sx) byl detekován u P. vulgare (2C= = 28,00 +/- 0,32 pg), P. xmantoniae (2C= = 37,18 +/- 0,38 pg) a P. interjectum (2C= = 45,24 +/- 0,31 pg) na průtokovém cytometru PAS Partec GmbH (barvení PI / standard Vicia faba, 2C= = 26,9 pg). Relativní obsah jaderné DNA byl zjišťován ve směsi všech tří taxonů (+/- Sx): P. vulgare : P. xmantoniae = 0,746 +/- 0,005; P. vulgare : P. interjectum = 0,595 +/- 0,001; P. xmantoniae : P. vulgare = 1,340 +/- 0,008; P. xmantoniae : P. interjectum = 0,797 +/- 0,005; P. interjectum : P. vulgare = 1,681 +/- 0,003; P. interjectum : P. xmantoniae = 1,255 +/- 0,008. Podle PI/DAPI indexu lze předpokládat, že poměr AT/GC (resp. zastoupení heterochromatinu) je u P. xmantoniae 1,05x větší než u P. vulgare a u P. interjectum 1,08x větší než u P. vulgare.

Links

• MSM 143100010, plan (intention): Name: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministry of Education, Youth and Sports of the CR, Spatiotemporal biodiversity dynamics in ecosystems of Central Europe