c-Fos but not v-Fos protein induces programmed cell death of v-myb-transformed monoblasts

ZAHRADNÍČKOVÁ, Eva, Karel SOUČEK, Sabina ŠEVČÍKOVÁ, Jana ŠMARDOVÁ and Jan ŠMARDA. c-Fos but not v-Fos protein induces programmed cell death of v-myb-transformed monoblasts. Cellular and Molecular Life Sciences. Bäsel: Birkhaeuser Verlag, 2003, vol. 60, No 12, p. 2736-2748. ISSN 1420-682X.

Basic information

• Original name: c-Fos but not v-Fos protein induces programmed cell death of v-myb-transformed monoblasts
• Authors: ZAHRADNÍČKOVÁ, Eva (203 Czech Republic), Karel SOUČEK (203 Czech Republic), Sabina ŠEVČÍKOVÁ (203 Czech Republic), Jana ŠMARDOVÁ (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor).
• Edition: Cellular and Molecular Life Sciences, Bäsel, Birkhaeuser Verlag, 2003, 1420-682X.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 4.995
• RIV identification code: RIV/00216224:14310/03:00008450
• Organization unit: Faculty of Science
• UT WoS: 000187503800015
• Keywords in English: Fos; Jun; AP-1; v-Myb; apoptosis; differentiation; proliferation; cell cycle
• Tags: AP-1, apoptosis, cell cycle, differentiation, Fos, Jun, proliferation, v-Myb

Abstract

c-Fos and v-Fos belong to a group of proteins forming the transcription factor AP-1 that is important for differentiation, proliferation and apoptosis in multiple cell types. In this study, we examined the role of c-Fos and v-Fos proteins in v-myb-transformed BM2 monoblats. We show that while the v-Fos protein prolongs the G0G1 phase of the BM2 cell cycle, c-Fos leaves the cell cycle unaffected and, rather, induces programmed cell death. The apoptosis promoting activity of the c-Fos protein is markedly enhanced in cells cultivated under serum-free conditions. c-Fos-induced apoptosis occurred in the presence of Bcl-2 and was not dependent on the transcription activation function of the c-Fos protein. No differentiation-promoting activity of the Fos proteins was observed. The effects of Fos proteins on BM2 cells differ from those induced by Jun proteins, suggesting differential roles of individual components of the AP-1 transcription factor in regulation essential cellular processes.

Links

• GA301/01/0040, research and development project: Name: Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

Investor: Czech Science Foundation, The Role of Fos and Jun proteins in RA-driven monocytic differentiation

• GA301/03/1055, research and development project: Name: Studium molekulárních mechanismů způsobujících supresi v-Myb s využitím přístupů proteomiky

Investor: Czech Science Foundation, Study of molecular mechanisms causing v-Myb suppression using proteomics-based approach

• MSM 143100008, plan (intention): Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions