The stereoisomers of several 1,3-dipolar cycloadducts were analysed without previous preparative separation, by HPLC on chiral nonracemic sorbents. This technique contributed significantly to the chemical purity checking, to the determination of the number of stereoisomers present, to the measurement of their relative proportions and to their characterization by retention factors and the circular dichroism (CD) properties. The detection with a CD instrument, in addition to a usual UV photometer, was carried out either at a fixed wavelength or by stopping the chromatographic flow, using a CD spectrum between 220 and 420 nm. The method presented is proposed as a supplementary analytical tool in organic stereochemistry.