J 2004

Electrophoretically mediated microanalysis (Review)

VYTISKOVÁ, Soňa, Sigrid VAN DYCK, Ann VAN SCHEPDAEL, Jos HOOGMARTENS, Zdeněk GLATZ et. al.

Basic information

Original name

Electrophoretically mediated microanalysis (Review)

Name in Czech

Elektroforeticky zprostředkovaná mirkoanalýza.

Authors

VYTISKOVÁ, Soňa (203 Czech Republic), Sigrid VAN DYCK (56 Belgium), Ann VAN SCHEPDAEL (56 Belgium), Jos HOOGMARTENS (56 Belgium) and Zdeněk GLATZ (203 Czech Republic, guarantor)

Edition

Journal of Chromatography A, Netherlands, Elsevier, 2004, 0021-9606

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.105

RIV identification code

RIV/00216224:14310/04:00009927

Organization unit

Faculty of Science

UT WoS

000220121200026

Keywords in English

Capillary electrophoresis; enzymes; EMMA
Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Abstract

V originále

This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.

In Czech

Jedná se o přehledný článek věnovaný elektroforeticky zprostředkované mirkoanalýze.

Links

GA203/03/1125, research and development project
Name: Využití kapilární zónové elektroforézy pro studium enzymů
Investor: Czech Science Foundation, Application of capillary zone electrophoresis for the study of enzymes
MSM 143100005, plan (intention)
Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism