2004
Cleavage of double stranded plasmid DNA by lanthanide complexes
RITTICH, Bohuslav, Alena ŠPANOVÁ, Martin FALK, Milan BENEŠ, Martin HRUBÝ et. al.Basic information
Original name
Cleavage of double stranded plasmid DNA by lanthanide complexes
Name in Czech
Štěpení dvouřetězcové DNA pomocí lanthanidových komplexů.
Authors
RITTICH, Bohuslav (203 Czech Republic, guarantor), Alena ŠPANOVÁ (203 Czech Republic), Martin FALK (203 Czech Republic), Milan BENEŠ (203 Czech Republic) and Martin HRUBÝ (203 Czech Republic)
Edition
Journal of Chromatography B, Amsterdam, Elsevier, 2004, 1570-0232
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
Genetics and molecular biology
Country of publisher
Netherlands
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 2.176
RIV identification code
RIV/00216224:14310/04:00011380
Organization unit
Faculty of Science
UT WoS
000187954500023
Keywords in English
Cleavage; Lanthanide icomplexes; Plasmid DNA pBR322
Tags
Reviewed
Changed: 23/11/2006 08:47, doc. Ing. Bohuslav Rittich, CSc.
V originále
The use of free lanthanide ions and their complexes for plasmid DNA pBR322 and chromosomal DNA was studied. Plasmid pBR322 DNA was treated by lanthanide chlorides (Eu3+, La3+, Nd3+, Pr3+, Gd3+ ) in HEPES buffer (pH 7.0, 7.5 and 8.0) at different temperatures. The formation of linear and nicked plasmid forms was investigated depending on the reaction conditions. Heterogeneous lanthanide complexes of ethylenediamine tetraacetic acid (EDTA) immobilized on insoluble methacrylate support and iminodiacetic acid (IDA) immobilized on styrene support were used as catalysts plasmid for DNA pBR322 cleavage, too. The temperature of reaction mixture had substantial influence on cleavage rate. The precipitation of DNA occured during the measurement of interactions between chromosomal DNA and La3+ ions.
In Czech
Bylo studováno použití volných lanthanidových iontů a jejich komplexů ke štěpení plazmidové pBR322 a chromosomální DNA. Plazmidová pBR322 DNA byla štěpena při různých teplotách chloridy lanthanidů (Eu3+, La3+, Nd3+, Pr3+, Gd3+ ) v HEPES pufru (pH 7,0; 7,5 and 8,0). Byla studována tvorba plazmidových forem (linearizované a s jednořetězcovým zlomem)v závislosti na použitých reakčních podmínkách. Jako katalyzátory při štěpení plazmidové pBR322 DNA byly rovněž použity heterogenní lanthanidové komplexy ethylendiamino tetraoctové kyseliny (EDTA),imobilizované na nerozpustném methakrylátovém nosiči a iminodioctová kyselina (IDA), imobilizovaná na styrénovém nosiči.
Links
| GA202/02/1361, research and development project |
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