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@article{493103, author = {Rittich, Bohuslav and Španová, Alena and Falk, Martin and Beneš, Milan and Hrubý, Martin}, article_location = {Amsterdam}, article_number = {1-2}, keywords = {Cleavage; Lanthanide icomplexes; Plasmid DNA pBR322}, language = {eng}, issn = {1570-0232}, journal = {Journal of Chromatography B}, title = {Cleavage of double stranded plasmid DNA by lanthanide complexes}, volume = {800}, year = {2004} }
TY - JOUR ID - 493103 AU - Rittich, Bohuslav - Španová, Alena - Falk, Martin - Beneš, Milan - Hrubý, Martin PY - 2004 TI - Cleavage of double stranded plasmid DNA by lanthanide complexes JF - Journal of Chromatography B VL - 800 IS - 1-2 SP - 169-173 EP - 169-173 PB - Elsevier SN - 15700232 KW - Cleavage KW - Lanthanide icomplexes KW - Plasmid DNA pBR322 N2 - The use of free lanthanide ions and their complexes for plasmid DNA pBR322 and chromosomal DNA was studied. Plasmid pBR322 DNA was treated by lanthanide chlorides (Eu3+, La3+, Nd3+, Pr3+, Gd3+ ) in HEPES buffer (pH 7.0, 7.5 and 8.0) at different temperatures. The formation of linear and nicked plasmid forms was investigated depending on the reaction conditions. Heterogeneous lanthanide complexes of ethylenediamine tetraacetic acid (EDTA) immobilized on insoluble methacrylate support and iminodiacetic acid (IDA) immobilized on styrene support were used as catalysts plasmid for DNA pBR322 cleavage, too. The temperature of reaction mixture had substantial influence on cleavage rate. The precipitation of DNA occured during the measurement of interactions between chromosomal DNA and La3+ ions. ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Martin FALK, Milan BENEŠ and Martin HRUBÝ. Cleavage of double stranded plasmid DNA by lanthanide complexes. \textit{Journal of Chromatography B}. Amsterdam: Elsevier, 2004, vol.~800, 1-2, p.~169-173. ISSN~1570-0232.
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