The Escherichia fergusonii iucABCD iutA genes are located
within a longer chromosomal region similar to pathogenicity
islands.

J 2003

The Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands.

ŠMAJS, David, Jan ŠMARDA and G.M. WEINSTOCK

Basic information

Original name

The Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands.

Name in Czech

Geny iucABCD iutA Escherichia fergusonii jsou lokalizovány uvnitř delší oblasti chromozomu podobné ostrovům patogenity.

Authors

ŠMAJS, David (203 Czech Republic, guarantor), Jan ŠMARDA (203 Czech Republic) and G.M. WEINSTOCK (840 United States of America)

Edition

Folia Microbiologica, 2003, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.857

Organization unit

Faculty of Medicine

UT WoS

000183030200002

Keywords (in Czech)

Escherichia fergusonii;aerobactin

Keywords in English

Escherichia fergusonii;aerobactin

Abstract

ORIG CZ

V originále

Three strains of Escherichia fergusonii (EF873, EF1496, EF939) of 50 strains tested produced the hydroxamate siderophore aerobactin. Screening of a cosmid library of the strain EF873 chromosomal DNA (in aerobactin nonproducing Escherichia coli VCS257) for aerobactin production identified iucABCD and iutA gene orthologues. The predicted IucABCD and IutA proteins showed 59-65% identity to the corresponding proteins of Shigella flexneri and E. coli. Aerobactin molecules synthesized by E. fergusonii and E. coli strains stimulated growth of aerobactin indicator strains harboring either E. coli or E. fergusonii iutA genes. In the 12 kb upstream and 17 kb downstream regions of the iuc and iut genes, 20 additional ORFs were identified. Their gene products showed homology to proteins from E. coli, S. flexneri, Klebsiella aerogenes, Pseudomonas aeruginosa and Vibrio cholerae. Probes recognizing DNA sequences from a region of more than 25 kb, which included the iucABCD and iutA genes, hybridized with chromosomal DNA of two aerobactin-producing strains (EF873 and EF939), but not with other nonproducing E. fergusonii strains tested. These data, together with the genetic organization of this region, suggest that E. fergusonii iucABCD iutA genes are a portion of a larger segment of DNA similar to pathogenicity islands of other bacteria.

In Czech

Tři kmeny Escherichia fergusonii (EF873, EF1496, EF939) z padesáti testovaných kmenů produkovaly aerobactin.

Citovat

ŠMAJS, David, Jan ŠMARDA and G.M. WEINSTOCK. The Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands. (he Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands.). Folia Microbiologica. 2003, 48/2003, No 48, p. 139-147. ISSN 0015-5632.

@article{493207,
   author = {Šmajs, David and Šmarda, Jan and Weinstock, G.M.},
   article_number = {48},
   keywords = {Escherichia fergusonii;aerobactin},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {The Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands.},
   volume = {48/2003},
   year = {2003}
}

TY  - JOUR
ID  - 493207
AU  - Šmajs, David - Šmarda, Jan - Weinstock, G.M.
PY  - 2003
TI  - The Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands.
JF  - Folia Microbiologica
VL  - 48/2003
IS  - 48
SP  - 139-147
EP  - 139-147
SN  - 00155632
KW  - Escherichia fergusonii;aerobactin
N2  - Three strains of Escherichia fergusonii (EF873, EF1496, EF939) of 50 strains tested produced the hydroxamate siderophore aerobactin. Screening of a cosmid library of the strain EF873 chromosomal DNA (in aerobactin nonproducing Escherichia coli VCS257) for aerobactin production identified iucABCD and iutA gene orthologues. The predicted IucABCD and IutA proteins showed 59-65% identity to the corresponding proteins of Shigella flexneri and E. coli. Aerobactin molecules synthesized by E. fergusonii and E. coli strains stimulated growth of aerobactin indicator strains harboring either E. coli or E. fergusonii iutA genes. In the 12 kb upstream and 17 kb downstream regions of the iuc and iut genes, 20 additional ORFs were identified. Their gene products showed homology to proteins from E. coli, S. flexneri, Klebsiella aerogenes, Pseudomonas aeruginosa and Vibrio cholerae. Probes recognizing DNA sequences from a region of more than 25 kb, which included the iucABCD and iutA genes, hybridized with chromosomal DNA of two aerobactin-producing strains (EF873 and EF939), but not with other nonproducing E. fergusonii strains tested. These data, together with the genetic organization of this region, suggest that E. fergusonii iucABCD iutA genes are a portion of a larger segment of DNA similar to pathogenicity islands of other bacteria.
ER  -

ŠMAJS, David, Jan ŠMARDA and G.M. WEINSTOCK. The Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands. (he Escherichia fergusonii iucABCD iutA genes are located within a longer chromosomal region similar to pathogenicity islands.). \textit{Folia Microbiologica}. 2003, 48/2003, No~48, p.~139-147. ISSN~0015-5632.

Detailed Information on Publication Record