PETROVÁ, Kateřina, Alena ŠPANOVÁ and Bohuslav RITTICH. Testování vlivu magnetických nosičů na bázi celulózy na průběh PCR (Testing of cellulose based magnetic carriers on the PCR course). In VIII. Pracovní setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita v Brně, 2004, p. 63. ISBN 80-210-3321-5.
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Basic information
Original name Testování vlivu magnetických nosičů na bázi celulózy na průběh PCR
Name in Czech Testování vlivu magnetických nosičů na bázi celulózy na průběh PCR
Name (in English) Testing of cellulose based magnetic carriers on the PCR course
Authors PETROVÁ, Kateřina (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic, guarantor) and Bohuslav RITTICH (203 Czech Republic).
Edition Brno, VIII. Pracovní setkání biochemiků a molekulárních biologů, p. 63-63, 2004.
Publisher Masarykova univerzita v Brně
Other information
Original language Czech
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/04:00019740
Organization unit Faculty of Science
ISBN 80-210-3321-5
Keywords in English Cellulose; magnetic carriers; Bifidobacterium; PCR
Tags Bifidobacterium, cellulose, magnetic carriers, PCR
Changed by Changed by: doc. Ing. Bohuslav Rittich, CSc., učo 1639. Changed: 11/6/2008 14:30.
Abstract (in English)
Influence of 8 magnetic carriers, including components used in their synthesis, on the PCR course was investigated. PCR primers Pbi F1 and Pbi R2 specific to genus Bifidobacterium were used. Specific PCR product (914 bp) was detected using agarose gel electrophoresis. Results of PCR were compared with positive controls. DNA of four different concentrations isolated from Bifidobacterium cells was used as positive control(it was added to PCR mixture without magnetic carriers).
Links
GA202/02/1361, research and development projectName: Využití magnetických nosičů s imobilizovanými selektivními ligandy v DNA diagnostice
Investor: Czech Science Foundation, The use of magnetic carriers with immobilized selective ligands in DNA diagnostics
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