Detailed Information on Publication Record
2003
Tumor suppressive effects of the p53 protein in v-myb-transformed monoblasts BM2
NAVRÁTILOVÁ, Jarmila, Bořivoj VOJTĚŠEK and Jan ŠMARDABasic information
Original name
Tumor suppressive effects of the p53 protein in v-myb-transformed monoblasts BM2
Name (in English)
Tumor suppressive effects of the p53 protein in v-myb-transformed monoblasts BM2
Authors
NAVRÁTILOVÁ, Jarmila (203 Czech Republic), Bořivoj VOJTĚŠEK (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor)
Edition
Vyškov, Memory in Living Systems, p. 65-65, 2003
Publisher
Czechoslovak Biological Society
Other information
Language
Czech
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/03:00009568
Organization unit
Faculty of Science
ISBN
80-210-3264-2
Keywords in English
p53; Myb; proliferation
Tags
Změněno: 22/4/2004 17:04, prof. RNDr. Jan Šmarda, CSc.
V originále
The v-myb oncogene of AMV causes rapid fatal monoblastic leukemia in chickens. In order to explore the ways to suppress transforming capabilities of the v-Myb we ectopically expressed p53 cDNA in AMV v-myb-transformed chicken monoblasts BM2. p53 can induce expression of multiple genes involved in control of cellular proliferation and apoptosis. Usually, the intracellular concentration of p53 protein is maintained at a very low level. However, DNA damage and other stress stimuli stabilize p53 increasing its cellular concentration. We transfected BM2 cells with human p53 cDNA under control inducible promoter and purified clones of stable transfectants. Stability of human p53 protein in this chicken cells was up-regulated by treatment with roscovitine and adriamycine. p53 did not arrest BM2 cell cycle but it increased apoptosis. Interestingly, induction of apoptosis occurred in p53-expressing BM2 cells in the absence of activation of bax, mdm2 and p21WAF/Cip1.
In English
The v-myb oncogene of AMV causes rapid fatal monoblastic leukemia in chickens. In order to explore the ways to suppress transforming capabilities of the v-Myb we ectopically expressed p53 cDNA in AMV v-myb-transformed chicken monoblasts BM2. p53 can induce expression of multiple genes involved in control of cellular proliferation and apoptosis. Usually, the intracellular concentration of p53 protein is maintained at a very low level. However, DNA damage and other stress stimuli stabilize p53 increasing its cellular concentration. We transfected BM2 cells with human p53 cDNA under control inducible promoter and purified clones of stable transfectants. Stability of human p53 protein in this chicken cells was up-regulated by treatment with roscovitine and adriamycine. p53 did not arrest BM2 cell cycle but it increased apoptosis. Interestingly, induction of apoptosis occurred in p53-expressing BM2 cells in the absence of activation of bax, mdm2 and p21WAF/Cip1.
Links
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