LOCHMAN, Jan, Vladimír MIKEŠ, Omar ŠERÝ and Libor JANKOVSKÝ. Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography. Mycological Research. Cambridge (U.K.): Cambridge University Press, 2004, vol. 108, No 10, p. 1153-1161. ISSN 0953-7562.
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Basic information
Original name Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography
Name in Czech Determinace variability ITS regionu v rámci ribozomální DNA václavek pomocí PCR a HPLC
Authors LOCHMAN, Jan (203 Czech Republic), Vladimír MIKEŠ (203 Czech Republic, guarantor), Omar ŠERÝ (203 Czech Republic) and Libor JANKOVSKÝ (203 Czech Republic).
Edition Mycological Research, Cambridge (U.K.), Cambridge University Press, 2004, 0953-7562.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.130
RIV identification code RIV/00216224:14310/04:00010027
Organization unit Faculty of Science
UT WoS 000224794900004
Keywords in English Armillaria; PCR; fungi
Tags Armillaria, fungi, PCR
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Omar Šerý, Ph.D., učo 18120. Changed: 1/3/2007 17:10.
Abstract
We analysed 40 isolates of species A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens mostly collected in Czech Republic by PCR-RFLP.Restriction fragments were analysed by ion-exchange high performance liquid chromatography that proved to be more useful, more sensible and less time consuming than classical electrophoresis on agarose gel.The cluster analysis based on the restrictions patterns of restriction endonucleases AluI and HinfI divided the analysed species into three groups.
Abstract (in Czech)
Analyzovali jsme celkem 40 izolátů druhů A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae a A. tabescens pomocí PCr a následné restrikční anlýzy. Restrikční fragenty byly analyzovány pomocí HPLC kolony, což zvýšilo senzitivitu, přesnost, rychlost a snížilo cenu detekce.
Links
MSM 143100005, plan (intention)Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism
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