Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography

LOCHMAN, Jan, Vladimír MIKEŠ, Omar ŠERÝ and Libor JANKOVSKÝ. Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography. Mycological Research. Cambridge (U.K.): Cambridge University Press, 2004, vol. 108, No 10, p. 1153-1161. ISSN 0953-7562.

Basic information

Original name

Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography

Name in Czech

Determinace variability ITS regionu v rámci ribozomální DNA václavek pomocí PCR a HPLC

Authors

LOCHMAN, Jan (203 Czech Republic), Vladimír MIKEŠ (203 Czech Republic, guarantor), Omar ŠERÝ (203 Czech Republic) and Libor JANKOVSKÝ (203 Czech Republic)

Edition

Mycological Research, Cambridge (U.K.), Cambridge University Press, 2004, 0953-7562

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.130

RIV identification code

RIV/00216224:14310/04:00010027

Organization unit

Faculty of Science

UT WoS

000224794900004

Keywords in English

Armillaria; PCR; fungi

Tags

Armillaria, fungi, PCR

Tags

International impact, Reviewed

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Abstract

V originále

We analysed 40 isolates of species A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens mostly collected in Czech Republic by PCR-RFLP.Restriction fragments were analysed by ion-exchange high performance liquid chromatography that proved to be more useful, more sensible and less time consuming than classical electrophoresis on agarose gel.The cluster analysis based on the restrictions patterns of restriction endonucleases AluI and HinfI divided the analysed species into three groups.

In Czech

Analyzovali jsme celkem 40 izolátů druhů A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae a A. tabescens pomocí PCr a následné restrikční anlýzy. Restrikční fragenty byly analyzovány pomocí HPLC kolony, což zvýšilo senzitivitu, přesnost, rychlost a snížilo cenu detekce.

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Links

MSM 143100005, plan (intention)

Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism