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@article{555073,
author = {Vacek, Jan and Andrysík, Zdeněk and Trnková, Libuše and Kizek, René},
article_location = {Germany, Weinheim},
article_number = {16},
keywords = {Azidothymidine (AZT); Square wave voltammetry; Hanging mercury drop electrode (HMDE); DNA; Albumin; Urine; Serum; Whole blood; Keratinocytes (HaCaT line).},
language = {eng},
issn = {1040-0397},
journal = {Electroanalysis},
title = {Determination of azidothymidine - an antiproliferative and virostatic drug by square wave voltammetry},
volume = {2004},
year = {2004}
}
TY - JOUR
ID - 555073
AU - Vacek, Jan - Andrysík, Zdeněk - Trnková, Libuše - Kizek, René
PY - 2004
TI - Determination of azidothymidine - an antiproliferative and virostatic drug by square wave voltammetry
JF - Electroanalysis
VL - 2004
IS - 16
SP - 224-230
EP - 224-230
PB - Wiley-VCH Verlag GmbH & Co.
SN - 10400397
KW - Azidothymidine (AZT)
KW - Square wave voltammetry
KW - Hanging mercury drop electrode (HMDE)
KW - DNA
KW - Albumin
KW - Urine
KW - Serum
KW - Whole blood
KW - Keratinocytes (HaCaT line).
N2 - The azido-deoxythymidine (AZT, Zidovudine) is an antiproliferative and virostatic drug widely used in human immunodeficiency virus type 1 (HIV-1) infection treatment. With respect to side effects of high doses and a short half-life of AZT, a fast and simple detection method for this agent could be helpful. The aim of our study was to determine AZT levels in natural samples (urine, serum, whole blood, and cell cultures, such as the HaCaT line of keratinocytes) without their mineralization and/or purification, by means of electrochemical methods using hanging mercury drop electrode (HMDE). On this electrode, AZT undergoes irreversible reduction at the peak potential near Ep -1.1 V (vs. Ag/AgCl/3M KCl). Reduction AZT signals were measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV), square wave voltammetry (SWV), and constant current chronopotentiometric stripping analysis (CPSA). In phosphate buffer (pH 8) the SWV yielded the best AZT signal with the detection limit of 1 nM. The determination of AZT concentration in biological materials is affected by electroactive components, such as proteins and DNA. For monitoring the influence of these compounds, AZT reduction was performed in the presence of 10 mg/mL calf thymus ssDNA and/or 100 mg/mL bovine serum albumin. In these cases, the detection limit increased to 0.25 mM. Also studied was the AZT concentration in keratinocyte cells was followed during cell cultivation. It has been shown that the SWV may be considered as a useful tool for the determination of AZT concentration in cell cultures, and for monitoring AZT pharmacokinetics.
ER -
VACEK, Jan, Zdeněk ANDRYSÍK, Libuše TRNKOVÁ and René KIZEK. Determination of azidothymidine - an antiproliferative and virostatic drug by square wave voltammetry. \textit{Electroanalysis}. Germany, Weinheim: Wiley-VCH Verlag GmbH \&{} Co., 2004, vol.~2004, No~16, p.~224-230. ISSN~1040-0397.
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