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@article{555077,
author = {Trnková, Libuše and Kizek, René and Vacek, Jan},
article_location = {The Netherlands},
article_number = {63},
keywords = {Azidothymidine (AZT); Square wave voltammetry (SWV); Linear sweep voltammetry (LSV); Elimination voltammetry with linear scan (EVLS); Hanging mercury drop electrode (HMDE); oligonucleotides; PCR product; chromosomal DNA},
language = {eng},
issn = {1567-5394},
journal = {Bioelectrochemistry},
title = {Square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA},
volume = {2004},
year = {2004}
}
TY - JOUR
ID - 555077
AU - Trnková, Libuše - Kizek, René - Vacek, Jan
PY - 2004
TI - Square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA
JF - Bioelectrochemistry
VL - 2004
IS - 63
SP - 31-36
EP - 31-36
PB - Elsevier
SN - 15675394
KW - Azidothymidine (AZT)
KW - Square wave voltammetry (SWV)
KW - Linear sweep voltammetry (LSV)
KW - Elimination voltammetry with linear scan (EVLS)
KW - Hanging mercury drop electrode (HMDE)
KW - oligonucleotides
KW - PCR product
KW - chromosomal DNA
N2 - Azidothymidine (AZT, 3-azido-3-deoxythymidine, Zidovudine, Retrovir) is an approved and widely used antiretroviral drug for the treatment of human immunodeficiency virus (HIV) infection. Dynamic electrochemical methods, have been employed for the fast and inexpensive determination of this drug in natural samples. The electrochemical signal of AZT, resulting from the reduction of azido group, was studied by square wave voltammetry (SWV), linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) using a hanging mercury drop electrode (HMDE). This paper explores the possibility of determining AZT in the presence of native (dsDNA) or denatured calf thymus DNA (ssDNA), and/or some synthetic oligodeoxynucleotides (ODNs). The detection limit of AZT in the absence and in the presence of ssDNA (10 mg/mL) is 1 nM and 250 nM, respectively. It was found that the signal of AZT is not substantially affected by the presence of DNA. We can therefore assume that the electrons are transferred through the adsorption layer of nucleic acids. By using the elimination procedure, both irreversible reduction signals of AZT and DNA are augmented. Moreover, the elimination signal in the peak-counterpeak form may indicate the adsorption of the analytes on the electrode surface preceding an electron transfer.
ER -
TRNKOVÁ, Libuše, René KIZEK and Jan VACEK. Square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2004, vol.~2004, No~63, p.~31-36. ISSN~1567-5394.
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