Detailed Information on Publication Record
2004
Využití katalytických reakcí na rtuťové elektrodě pro elektrochemické stanovení metalothioneinů
KIZEK, René, Jan VACEK, Libuše TRNKOVÁ, Bořivoj KLEJDUS, Ladislav HAVEL et. al.Basic information
Original name
Využití katalytických reakcí na rtuťové elektrodě pro elektrochemické stanovení metalothioneinů
Name (in English)
Utilization of catalytic reactions on mercury electrode for the electrochemical determination of metalothioneins
Authors
KIZEK, René (203 Czech Republic), Jan VACEK (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor), Bořivoj KLEJDUS (203 Czech Republic) and Ladislav HAVEL (203 Czech Republic)
Edition
Chem. listy, Praha, Česká společnost chemická, 2004, 0009-2770
Other information
Language
Czech
Type of outcome
Článek v odborném periodiku
Field of Study
10405 Electrochemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.348
RIV identification code
RIV/00216224:14310/04:00009640
Organization unit
Faculty of Science
Keywords in English
metalothioneins; electrochemical analysis;HMDE; catalytic signals;prenatrium wave; peak H;Brdicka reaction
Tags
Změněno: 21/5/2004 20:46, prof. RNDr. Libuše Trnková, CSc.
V originále
Metallothioneins (MTs) belong to a group of oligo- and polypeptides which play an important role in the metabolism of metals in animals, plants and microorganisms. The metabolic function of MTs consists not only in detoxication of an organism but also in the homeostasis of essential metals. This paper reports a novel approach to electroanalysis of MTs. The electrochemical determination of MTs is based on catalytic processes which proceed at very negative potentials on mercury electrodes (from -1.7 V to -1.9 V vs. Ag/AgCl/KCl). These processes accompanied by evolution of hydrogen from supporting electrolyte components, include the Heyrovsky presodium wave and/or Brdicka reaction. It was found that SH groups present in MTs are responsible for catalytic processes. The catalytic signal of a MT at nanomolar concentrations can be detected on mercury electrodes using potentiostatic electrochemical methods. The highest sensitivity in the determination of MTs was observed with a galvanostatic method such as derivative potentiometric stripping analysis (CPSA) which produces the peak H. The coupling of CPSA with the adsorptive transfer stripping technique (AdTS) allows the determination of MTs at a femtomole level in a low amount of sample (5 ěl). Our recent results obtained by AdTS + CPSA show that detection limits for particular MTs can be improved by adding [Co(NH3)6] Cl3 to the sample analyzed. This is probably due to the formation of a complex between [Co(NH3)6] Cl3 and MT. We think that the peak H can be used in physiological studies of metal metabolism.
In English
Metallothioneins (MTs) belong to a group of oligo- and polypeptides which play an important role in the metabolism of metals in animals, plants and microorganisms. The metabolic function of MTs consists not only in detoxication of an organism but also in the homeostasis of essential metals. This paper reports a novel approach to electroanalysis of MTs. The electrochemical determination of MTs is based on catalytic processes which proceed at very negative potentials on mercury electrodes (from -1.7 V to -1.9 V vs. Ag/AgCl/KCl). These processes accompanied by evolution of hydrogen from supporting electrolyte components, include the Heyrovsky presodium wave and/or Brdicka reaction. It was found that SH groups present in MTs are responsible for catalytic processes. The catalytic signal of a MT at nanomolar concentrations can be detected on mercury electrodes using potentiostatic electrochemical methods. The highest sensitivity in the determination of MTs was observed with a galvanostatic method such as derivative potentiometric stripping analysis (CPSA) which produces the peak H. The coupling of CPSA with the adsorptive transfer stripping technique (AdTS) allows the determination of MTs at a femtomole level in a low amount of sample (5 ěl). Our recent results obtained by AdTS + CPSA show that detection limits for particular MTs can be improved by adding [Co(NH3)6] Cl3 to the sample analyzed. This is probably due to the formation of a complex between [Co(NH3)6] Cl3 and MT. We think that the peak H can be used in physiological studies of metal metabolism.
Links
| GA203/02/0422, research and development project |
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| IAA1163201, research and development project |
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