VACEK, Jan, Jiří PETŘEK, René KIZEK, Ladislav HAVEL, Bořivoj KLEJDUS, Libuše TRNKOVÁ and František JELEN. Electrochemical determination of lead and glutathione in a plant cell culture. Bioelectrochemistry. The Netherlands: Elsevier, 2004, vol. 2004, No 63, p. 347-351. ISSN 1567-5394.
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Basic information
Original name Electrochemical determination of lead and glutathione in a plant cell culture
Name in Czech Electrochemická detekce olova a glutathionu v rostlinných buněčných kulturách
Authors VACEK, Jan (203 Czech Republic), Jiří PETŘEK (203 Czech Republic), René KIZEK (203 Czech Republic), Ladislav HAVEL (203 Czech Republic), Bořivoj KLEJDUS (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and František JELEN (203 Czech Republic).
Edition Bioelectrochemistry, The Netherlands, Elsevier, 2004, 1567-5394.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10405 Electrochemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.261
RIV identification code RIV/00216224:14310/04:00011181
Organization unit Faculty of Science
UT WoS 000221786900064
Keywords in English Lead; Pb(II)-EDTA; glutathione; metallothionein; Voltammetry; Mercury electrode; Brdicka reaction
Tags Brdicka reaction, Glutathione, lead, Mercury Electrode, metallothionein, Pb(II)-EDTA, Voltammetry
Changed by Changed by: prof. RNDr. Libuše Trnková, CSc., učo 1027. Changed: 15/2/2005 08:38.
Abstract
In this paper we established DPASV as the tool for the analysis of lead in plant cell culture. For the calibration procedure, lead in (Pb-EDTA) chelate has been used. The detection limit of lead was at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract. For the determination of cystein-rich peptides, voltammetry in differential mode in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used.
Abstract (in Czech)
Electrochemická detekce olova a glutathionu v rostlinných buněčných kulturách
Links
GA203/02/0422, research and development projectName: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí
Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
IAA1163201, research and development projectName: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin
Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
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