J 2004

Electrochemical determination of lead and glutathione in a plant cell culture

VACEK, Jan, Jiří PETŘEK, René KIZEK, Ladislav HAVEL, Bořivoj KLEJDUS et. al.

Basic information

Original name

Electrochemical determination of lead and glutathione in a plant cell culture

Name in Czech

Electrochemická detekce olova a glutathionu v rostlinných buněčných kulturách

Authors

VACEK, Jan (203 Czech Republic), Jiří PETŘEK (203 Czech Republic), René KIZEK (203 Czech Republic), Ladislav HAVEL (203 Czech Republic), Bořivoj KLEJDUS (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and František JELEN (203 Czech Republic)

Edition

Bioelectrochemistry, The Netherlands, Elsevier, 2004, 1567-5394

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.261

RIV identification code

RIV/00216224:14310/04:00011181

Organization unit

Faculty of Science

UT WoS

000221786900064

Keywords in English

Lead; Pb(II)-EDTA; glutathione; metallothionein; Voltammetry; Mercury electrode; Brdicka reaction
Změněno: 15/2/2005 08:38, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

In this paper we established DPASV as the tool for the analysis of lead in plant cell culture. For the calibration procedure, lead in (Pb-EDTA) chelate has been used. The detection limit of lead was at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract. For the determination of cystein-rich peptides, voltammetry in differential mode in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used.

In Czech

Electrochemická detekce olova a glutathionu v rostlinných buněčných kulturách

Links

GA203/02/0422, research and development project
Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí
Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
IAA1163201, research and development project
Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin
Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.