Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry
PREISLER, Jan, Patrik VRÁBEL, Jaromíra NOVÁKOVÁ, Michal SPĚŠNÝ, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Pavel KRÁSENSKÝ, Luděk EYER, Jiří DOŠKAŘ, Roman PANTŮČEK, František FORET and Karel KLEPÁRNÍK. Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry. In Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis. 2004. |
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Basic information | |
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Original name | Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry |
Name in Czech | Kapilární elektroforéza proteinů bakteriofága 812 kombinovaná s MALDI-hmotnostní spektrometrií |
Authors | PREISLER, Jan (203 Czech Republic, guarantor, belonging to the institution), Patrik VRÁBEL (203 Czech Republic, belonging to the institution), Jaromíra NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Michal SPĚŠNÝ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Hana KONEČNÁ (203 Czech Republic, belonging to the institution), Pavel KRÁSENSKÝ (203 Czech Republic, belonging to the institution), Luděk EYER (203 Czech Republic, belonging to the institution), Jiří DOŠKAŘ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), František FORET (203 Czech Republic) and Karel KLEPÁRNÍK (203 Czech Republic). |
Edition | Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis, 2004. |
Other information | |
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Original language | English |
Type of outcome | Conference abstract |
Field of Study | 10406 Analytical chemistry |
Country of publisher | Austria |
Confidentiality degree | is not subject to a state or trade secret |
WWW | URL |
RIV identification code | RIV/00216224:14310/04:00011440 |
Organization unit | Faculty of Science |
Keywords in English | Capillary Electrophoresis; Proteins; Bacteriophage |
Tags | Bacteriophage, Capillary electrophoresis, proteins |
Tags | International impact, Reviewed |
Changed by | Changed by: prof. Mgr. Jan Preisler, Ph.D., učo 45329. Changed: 11/4/2013 16:32. |
Abstract |
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The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification. |
Abstract (in Czech) |
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The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification. Anotace anglicky: The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification. |
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GA203/03/0515, research and development project | Name: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie |
Investor: Czech Science Foundation, Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry |
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