| SKLÁDAL, Petr, Carla SANTOS RICARDI, Hideko YAMANAKA a Paolo COSTA. Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus. Journal of Virological Methods. Amsterdam: Elsevier, 2004, roč. 117, č. 2, s. 145-151. ISSN 0166-0934. |
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@article{555475,
author = {Skládal, Petr and Santos Ricardi, Carla and Yamanaka, Hideko and Costa, Paolo},
article_location = {Amsterdam},
article_number = {2},
keywords = {Piezoelectric biosesnsor; DNA; Hepatitis C; PCR; Hybridization},
language = {eng},
issn = {0166-0934},
journal = {Journal of Virological Methods},
title = {Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus},
volume = {117},
year = {2004}
}
TY - JOUR
ID - 555475
AU - Skládal, Petr - Santos Ricardi, Carla - Yamanaka, Hideko - Costa, Paolo
PY - 2004
TI - Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus
JF - Journal of Virological Methods
VL - 117
IS - 2
SP - 145-151
EP - 145-151
PB - Elsevier
SN - 01660934
KW - Piezoelectric biosesnsor
KW - DNA
KW - Hepatitis C
KW - PCR
KW - Hybridization
N2 - The piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus in serum. The gold electrodes on either rough or smooth surface crystals were modified with a self-assembled monolayer of cystamine. After activation with glutaraldehyde, either avidin or streptavidin were immobilized and used for attachment of biotinylated DNA probes (4 different sequences). Thus prepared piezoelectric biosensors were used in a flow-through setup for direct monitoring of DNA resulting from the reverse transcriptase linked polymerase chain reaction (RT-PCR) amplification of the original viral RNA. The samples of patients with hepatitis C were analyzed and the results were compared with the standard RT-PCR procedure (Amplicor test kit of Roche, microwell format with spectrophotometric evaluation). The piezoelectric hybridization assay was completed in 10 min and the same sensing surface was suitable for repeated use.
ER -
SKLÁDAL, Petr, Carla SANTOS RICARDI, Hideko YAMANAKA a Paolo COSTA. Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus. \textit{Journal of Virological Methods}. Amsterdam: Elsevier, 2004, roč.~117, č.~2, s.~145-151. ISSN~0166-0934.
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