The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

NAVRÁTILOVÁ, Iva and Petr SKLÁDAL. The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy. Bioelectrochemistry. The Netherlands: Elsevier, 2004, vol. 62, No 1, p. 11-18. ISSN 1567-5394.

Basic information

• Original name: The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy
• Name in Czech: Imunosensor pro stanovení 2,4-dichlorofenoxyoctové kyseliny využívající elektrochemickou impedanční spektroskopii
• Authors: NAVRÁTILOVÁ, Iva (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor).
• Edition: Bioelectrochemistry, The Netherlands, Elsevier, 2004, 1567-5394.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.261
• RIV identification code: RIV/00216224:14310/04:00010107
• Organization unit: Faculty of Science
• UT WoS: 000221786300002
• Keywords in English: Impedance; Immunosensor; Herbicide
• Tags: Herbicide, immunosensor, Impedance

Abstract

Electrochemical impedance spectroscopy was evaluated for the direct determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Specific antibody against 2,4-D was immobilised onto different gold electrodes. Several methods of antibody immobilisation by covalent linkage to modified surface were studied. Self-assembled monolayers formed using thiocompounds as cystamine, 4-aminothiophenol (ATPh), 3,3'-dithiopropionic acid di(N-succinimidyl ester) (DTSP) and 11-mercaptoundecanoic acid (MUA) were chosen for the sensing surface activation. Three different sensor types were tested: screen-printed disc and finger-like structures and interdigitated array (IDA) electrodes produced by lithography. The measurements were carried out in a stationary arrangement and the reaction between hapten and the immobilised antibody was observed on-line. Changes of impedance parameters were evaluated and the best immobilisation technique (using 4-aminothiophenol) was chosen for further measurements. Impedance changes due to immunocomplex formation were evaluated and the possibility of direct monitoring of 2,4-D binding to the antibody was demonstrated at a fixed frequency.

Abstract (in Czech)

Pro přímé stanovení herbicidu 2,4-D byla využita elektroimpedanční spektroskopie. Protilátky proti 2,4-D byly navázány na zlaté elektrody použitím různých monovrstev thiosloučenin (MUA, DTSP, cystamin, aminothiofenol). Byly využity sensory připravené sítotiskem i litografickou technologií. Byla sledována změna impedance sensorů v přítomnosti analytu.

Links

• OC 518.30, research and development project: Name: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministry of Education, Youth and Sports of the CR, Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors