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@article{555477, author = {Navrátilová, Iva and Skládal, Petr}, article_location = {The Netherlands}, article_number = {1}, keywords = {Impedance; Immunosensor; Herbicide}, language = {eng}, issn = {1567-5394}, journal = {Bioelectrochemistry}, title = {The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy}, volume = {62}, year = {2004} }
TY - JOUR ID - 555477 AU - Navrátilová, Iva - Skládal, Petr PY - 2004 TI - The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy JF - Bioelectrochemistry VL - 62 IS - 1 SP - 11-18 EP - 11-18 PB - Elsevier SN - 15675394 KW - Impedance KW - Immunosensor KW - Herbicide N2 - Electrochemical impedance spectroscopy was evaluated for the direct determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Specific antibody against 2,4-D was immobilised onto different gold electrodes. Several methods of antibody immobilisation by covalent linkage to modified surface were studied. Self-assembled monolayers formed using thiocompounds as cystamine, 4-aminothiophenol (ATPh), 3,3'-dithiopropionic acid di(N-succinimidyl ester) (DTSP) and 11-mercaptoundecanoic acid (MUA) were chosen for the sensing surface activation. Three different sensor types were tested: screen-printed disc and finger-like structures and interdigitated array (IDA) electrodes produced by lithography. The measurements were carried out in a stationary arrangement and the reaction between hapten and the immobilised antibody was observed on-line. Changes of impedance parameters were evaluated and the best immobilisation technique (using 4-aminothiophenol) was chosen for further measurements. Impedance changes due to immunocomplex formation were evaluated and the possibility of direct monitoring of 2,4-D binding to the antibody was demonstrated at a fixed frequency. ER -
NAVRÁTILOVÁ, Iva and Petr SKLÁDAL. The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2004, vol.~62, No~1, p.~11-18. ISSN~1567-5394.
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