Detailed Information on Publication Record
2004
The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy
NAVRÁTILOVÁ, Iva and Petr SKLÁDALBasic information
Original name
The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy
Name in Czech
Imunosensor pro stanovení 2,4-dichlorofenoxyoctové kyseliny využívající elektrochemickou impedanční spektroskopii
Authors
NAVRÁTILOVÁ, Iva (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)
Edition
Bioelectrochemistry, The Netherlands, Elsevier, 2004, 1567-5394
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.261
RIV identification code
RIV/00216224:14310/04:00010107
Organization unit
Faculty of Science
UT WoS
000221786300002
Keywords in English
Impedance; Immunosensor; Herbicide
Tags
Změněno: 18/1/2005 10:23, prof. RNDr. Petr Skládal, CSc.
V originále
Electrochemical impedance spectroscopy was evaluated for the direct determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Specific antibody against 2,4-D was immobilised onto different gold electrodes. Several methods of antibody immobilisation by covalent linkage to modified surface were studied. Self-assembled monolayers formed using thiocompounds as cystamine, 4-aminothiophenol (ATPh), 3,3'-dithiopropionic acid di(N-succinimidyl ester) (DTSP) and 11-mercaptoundecanoic acid (MUA) were chosen for the sensing surface activation. Three different sensor types were tested: screen-printed disc and finger-like structures and interdigitated array (IDA) electrodes produced by lithography. The measurements were carried out in a stationary arrangement and the reaction between hapten and the immobilised antibody was observed on-line. Changes of impedance parameters were evaluated and the best immobilisation technique (using 4-aminothiophenol) was chosen for further measurements. Impedance changes due to immunocomplex formation were evaluated and the possibility of direct monitoring of 2,4-D binding to the antibody was demonstrated at a fixed frequency.
In Czech
Pro přímé stanovení herbicidu 2,4-D byla využita elektroimpedanční spektroskopie. Protilátky proti 2,4-D byly navázány na zlaté elektrody použitím různých monovrstev thiosloučenin (MUA, DTSP, cystamin, aminothiofenol). Byly využity sensory připravené sítotiskem i litografickou technologií. Byla sledována změna impedance sensorů v přítomnosti analytu.
Links
| OC 518.30, research and development project |
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