a 2002

The study of potentially pathogenic lipophilic yeast Malassezia pachydermatis

DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁ

Basic information

Original name

The study of potentially pathogenic lipophilic yeast Malassezia pachydermatis

Authors

DAVID, Marek (203 Czech Republic, belonging to the institution), Miroslav GABRIEL (203 Czech Republic, guarantor, belonging to the institution) and Marie KOPECKÁ (203 Czech Republic, belonging to the institution)

Edition

30nd Annual Conference on Yeasts, 2002

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/02:00011445

Organization unit

Faculty of Medicine

Keywords in English

lipophilic yeast; Malassezia pachydermatis; transmission electron microscopy; ultrastructure

Tags

Změněno: 29/3/2011 13:59, Mgr. Marek David, Ph.D.

Abstract

V originále

The potential human pathogen M. pachydermatis was studied to identify cytoskeleton as potential therapeutic target for antifungal agents. Light and transmission electron microscopy (TEM) confirmed the small cells (2,0-3,0 x 4,0-5,0 mm) and few intracellular structures. TEM confirmed the presence of thick multilayered cell wall that appeared to be a barrier for fluorescent antibodies. The higher concentration of detergent and lytical wall enzymes, the prolonged time of their and fluorescent antibodies applications seem necessary prerequisites to detect cytoskeleton. Preliminary results showed poor cytoskeletal structures. This report confirmed multilayered cell wall, the deep and helicoidal invaginations of plasma membrane and few intracellular structures. Cytoskeleton will be further studied by fluorescence and immunofluorescence and by TEM. The research was supported by grant No. 310/00/0391 of the Grant Agency of Czech Republic.

Links

GA310/00/0391, research and development project
Name: Cytoskelet u lidských patogenních kvasinkových mikroorganismů.
Investor: Czech Science Foundation, Cytoskeleton in human pathogenic yeast microorganisms