Differentiation of brown adipose tissue and biogenesis of thermogenic mitochondria in situ and in cell culture

HOUŠTĚK, Josef, Jan KOPECKÝ, Marie BAUDYŠOVÁ, Dagmar JANÍKOVÁ, Stanislav PAVELKA and Petr KLEMENT. Differentiation of brown adipose tissue and biogenesis of thermogenic mitochondria in situ and in cell culture. Biochimica et Biophysica Acta [IF=2.411 (1989); SCI #cit.<22>]. 1990, vol. 1018, p. 243-247. ISSN 0006-3002.

Basic information

Original name

Differentiation of brown adipose tissue and biogenesis of thermogenic mitochondria in situ and in cell culture

Authors

HOUŠTĚK, Josef, Jan KOPECKÝ, Marie BAUDYŠOVÁ, Dagmar JANÍKOVÁ, Stanislav PAVELKA and Petr KLEMENT

Edition

Biochimica et Biophysica Acta [IF=2.411 (1989); SCI #cit.<22>], 1990, 0006-3002

---

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

Biogenesis; brown adipose tissue; differentiation; mitochondrion; thermogenesis; uncoupling protein

Tags

Biogenesis, brown adipose tissue, differentiation, mitochondrion, thermogenesis, uncoupling protein

Tags

International impact, Reviewed

---

Abstract

V originále

Differentiation and biogenesis of mitochondria in brown adipose tissue (BAT) was studied in situ and in cell culture by Western blotting, enzyme activity measurements, [35S]methionine incorporation and immuno-fluorescence microscopy. The biogenesis was characterized by the sudden appearance and rapid increase of the uncoupling protein (UCP), increase of cytochrome oxidase (COX) and decrease of H+ -ATPase.