Detailed Information on Publication Record
2004
Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium
LUKÁŠOVÁ, Emilie, Stanislav KOZUBEK, Martin FALK, Michal KOZUBEK, Jan ŽALOUDÍK et. al.Basic information
Original name
Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium
Name in Czech
Topografie genetických lokusů v jádrech buněk kolorektálního karcionomu a přiléhající tkáně střevního epitelu
Authors
LUKÁŠOVÁ, Emilie (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Martin FALK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic, guarantor, belonging to the institution), Jan ŽALOUDÍK (203 Czech Republic, belonging to the institution), Václav VAGUNDA (203 Czech Republic) and Zdeněk PAVLOVSKÝ (203 Czech Republic, belonging to the institution)
Edition
Chromosoma, Berlin, Springer-Verlag, 2004, 0009-5915
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.714
RIV identification code
RIV/00216224:14330/04:00009648
Organization unit
Faculty of Informatics
UT WoS
000189275900002
Keywords in English
chromatin structure; colorectal carcinoma
Tags
International impact, Reviewed
Změněno: 24/8/2012 13:28, doc. RNDr. Martin Falk, Ph.D.
V originále
To determine the influence of increased gene expression and amplification in colorectal carcinoma on chromatin structure, the nuclear distances between pairs of bacterial artificial chromosome (BAC) clones with genomic separation from 800 to 29,000 kb were measured and compared between the tumor and parallel epithelial cells of six patients. The nuclear distances were measured between the loci in chromosomal bands 7p22.3-7p21.3; 7q35-7q36.3; 11p15.5-11p15.4; 20p13; 20p12.2; 20q11.21 and 20q12 where increased expression had been found in all types of colorectal carcinoma. The loci were visualized by three-dimensional fluorescence in situ hybridization using 22 BAC clones. Our results show that for short genomic separations, mean nuclear distance increases linearly with increased genomic separation. The results for some pairs of loci fell outside this linear slope, indicating the existence of different levels of chromatin folding. For the same genomic separations the nuclear distances were frequently shorter for tumor as compared with epithelial cells. Above the initial growing phase of the nuclear distances, a plateau phase was observed in both cell types where the increase in genomic separation was not accompanied by an increase in nuclear distance. The ratio of the mean nuclear distances between the corresponding loci in tumor and epithelium cells decreases with increasing amplification of loci. Our results further show that the large-scale chromatin folding might differ for specific regions of chromosomes and that it is basically preserved in tumor cells in spite of the amplification of many loci.
In Czech
Článek pojednává o topografii genetických lokusů v jádrech buněk kolorektálního karcionomu a přiléhající tkáně střevního epitelu
Links
| GA202/02/0804, research and development project |
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| IAA1065203, research and development project |
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| IAA5004306, research and development project |
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| NC6987, research and development project |
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