Genome Size Variation in Central European Species of Cirsium
(Compositae) and their Natural Hybrids

J 2004

Genome Size Variation in Central European Species of Cirsium (Compositae) and their Natural Hybrids

BUREŠ, Petr, Yi-Feng WANG, Lucie HOROVÁ and Jan SUDA

Basic information

Original name

Genome Size Variation in Central European Species of Cirsium (Compositae) and their Natural Hybrids

Name in Czech

Velikost genomu u středoevropských druhů rodu Cirsium a jejich kříženců

Authors

BUREŠ, Petr (203 Czech Republic), Yi-Feng WANG (156 China), Lucie HOROVÁ (203 Czech Republic, guarantor) and Jan SUDA (203 Czech Republic)

Edition

Annals of Botany, Oxford, Oxford University Press, 2004, 0305-7364

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.262

RIV identification code

RIV/00216224:14310/04:00010225

Organization unit

Faculty of Science

UT WoS

000223894600004

Keywords in English

AT frequency; Asteraceae; base composition; C-value; DAPI; DNA content; Ellenberg's indicator values; flow cytometry; genome size; interspecific hybrids; propidium iodide; thistle

Abstract

ORIG CZ

V originále

Background and Aims: Nuclear DNA amounts of 12 diploid and one tetraploid taxa and 12 natural interspecific hybrids of Cirsium from 102 populations in the Czech Republic, Austria, Slovakia and Hungary were estimated. Methods: DAPI and PI flow cytometry were used. Key Results: 2C-values of diploid (2n = 34) species varied from 214 pg in C. heterophyllum to 360 pg in C. eriophorum (168-fold difference); the 2C value for the tetraploid C. vulgare was estimated at 554 pg. The DNA contents of hybrids were located between the values of their putative parents, although usually closer to the species with the smaller genome. Biennial species of Cirsium possessed larger nuclear DNA amounts than their perennial relatives. Genome size was negatively correlated with Ellenberg's indicator values for continentality and moisture and with eastern limits of distribution. A negative relationship was also detected between the genome size and the tendency to form natural interspecific hybrids. On the contrary, C-values positively corresponded with the spinyness (degree of spinosity). AT frequency ranged from 4838 % in C. eriophorum to 5175 % in C. arvense. Significant intraspecific DNA content variation in DAPI sessions was detected in C. acaule (probably due to the presence of B-chromosomes), and in tetraploid C. vulgare. Only the diploid level was confirmed for the Pannonian C. brachycephalum, generally considered to be tetraploid. In addition, triploidy was discovered for the first time in C. rivulare. Conclusions: Considerable differences in nuclear DNA content exist among Central European species of Cirsium on the diploid level. Perennial soft spiny Cirsium species of wet habitats and continental distributions generally have smaller genomes. The hybrids of diploid species remain diploid, and their DNA content is smaller than the mean of the parents. Species with smaller genomes produce interspecific hybrids more frequently.

In Czech

Velikost genomu u středoevropských druhů rodu Cirsium a jejich kříženců.

Links

MSM 143100010, plan (intention)

Name: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministry of Education, Youth and Sports of the CR, Spatiotemporal biodiversity dynamics in ecosystems of Central Europe

Citovat

BUREŠ, Petr, Yi-Feng WANG, Lucie HOROVÁ and Jan SUDA. Genome Size Variation in Central European Species of Cirsium (Compositae) and their Natural Hybrids. Annals of Botany. Oxford: Oxford University Press, 2004, vol. 94, No 3, p. 353-363. ISSN 0305-7364.

@article{556990,
   author = {Bureš, Petr and Wang, YiandFeng and Horová, Lucie and Suda, Jan},
   article_location = {Oxford},
   article_number = {3},
   keywords = {AT frequency; Asteraceae; base composition; C-value; DAPI; DNA content; Ellenberg's indicator values; flow cytometry; genome size; interspecific hybrids; propidium iodide; thistle},
   language = {eng},
   issn = {0305-7364},
   journal = {Annals of Botany},
   title = {Genome Size Variation in Central European Species of Cirsium (Compositae) and their Natural Hybrids},
   url = {http://aob.oupjournals.org/cgi/content/full/mch151?ijkey=15tWtQ7wJE5kQ&keytype=ref},
   volume = {94},
   year = {2004}
}

TY  - JOUR
ID  - 556990
AU  - Bureš, Petr - Wang, Yi-Feng - Horová, Lucie - Suda, Jan
PY  - 2004
TI  - Genome Size Variation in Central European Species of Cirsium (Compositae) and their Natural Hybrids
JF  - Annals of Botany
VL  - 94
IS  - 3
SP  - 353-363
EP  - 353-363
PB  - Oxford University Press
SN  - 03057364
KW  - AT frequency
KW  - Asteraceae
KW  - base composition
KW  - C-value
KW  - DAPI
KW  - DNA content
KW  - Ellenberg's indicator values
KW  - flow cytometry
KW  - genome size
KW  - interspecific hybrids
KW  - propidium iodide
KW  - thistle
UR  - http://aob.oupjournals.org/cgi/content/full/mch151?ijkey=15tWtQ7wJE5kQ&keytype=ref
N2  - Background and Aims: Nuclear DNA amounts of 12 diploid and one tetraploid taxa and 12 natural interspecific hybrids of Cirsium from 102 populations in the Czech Republic, Austria, Slovakia and Hungary were estimated. Methods: DAPI and PI flow cytometry were used. Key Results: 2C-values of diploid (2n = 34) species varied from 214 pg in C. heterophyllum to 360 pg in C. eriophorum (168-fold difference); the 2C value for the tetraploid C. vulgare was estimated at 554 pg. The DNA contents of hybrids were located between the values of their putative parents, although usually closer to the species with the smaller genome. Biennial species of Cirsium possessed larger nuclear DNA amounts than their perennial relatives. Genome size was negatively correlated with Ellenberg's indicator values for continentality and moisture and with eastern limits of distribution. A negative relationship was also detected between the genome size and the tendency to form natural interspecific hybrids. On the contrary, C-values positively corresponded with the spinyness (degree of spinosity). AT frequency ranged from 4838 % in C. eriophorum to 5175 % in C. arvense. Significant intraspecific DNA content variation in DAPI sessions was detected in C. acaule (probably due to the presence of B-chromosomes), and in tetraploid C. vulgare. Only the diploid level was confirmed for the Pannonian C. brachycephalum, generally considered to be tetraploid. In addition, triploidy was discovered for the first time in C. rivulare. Conclusions: Considerable differences in nuclear DNA content exist among Central European species of Cirsium on the diploid level. Perennial soft spiny Cirsium species of wet habitats and continental distributions generally have smaller genomes. The hybrids of diploid species remain diploid, and their DNA content is smaller than the mean of the parents. Species with smaller genomes produce interspecific hybrids more frequently.
ER  -

BUREŠ, Petr, Yi-Feng WANG, Lucie HOROVÁ and Jan SUDA. Genome Size Variation in Central European Species of Cirsium (Compositae) and their Natural Hybrids. \textit{Annals of Botany}. Oxford: Oxford University Press, 2004, vol.~94, No~3, p.~353-363. ISSN~0305-7364.

Detailed Information on Publication Record