| BOUCHAL, Pavel, Petra PŘECECHTĚLOVÁ, Zbyněk ZDRÁHAL and Igor KUČERA. Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide. Proteomics. Weinheim: Wiley-VCH, 2004, vol. 4, No 9, p. 2662-2671. ISSN 1615-9853. |
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@article{557057,
author = {Bouchal, Pavel and Přecechtělová, Petra and Zdráhal, Zbyněk and Kučera, Igor},
article_location = {Weinheim},
article_number = {9},
keywords = {Paracoccus denitrificans; Protein database; Proteome; Terminal electron acceptor; Two-dimensional electrophoresis},
language = {eng},
issn = {1615-9853},
journal = {Proteomics},
title = {Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide},
url = {http://www.wiley-vch.de/contents/jc_2120/2004/pro0833_s.pdf},
volume = {4},
year = {2004}
}
TY - JOUR
ID - 557057
AU - Bouchal, Pavel - Přecechtělová, Petra - Zdráhal, Zbyněk - Kučera, Igor
PY - 2004
TI - Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide
JF - Proteomics
VL - 4
IS - 9
SP - 2662-2671
EP - 2662-2671
PB - Wiley-VCH
SN - 16159853
KW - Paracoccus denitrificans
KW - Protein database
KW - Proteome
KW - Terminal electron acceptor
KW - Two-dimensional electrophoresis
UR - http://www.wiley-vch.de/contents/jc_2120/2004/pro0833_s.pdf
N2 - Two-dimensional gel electrophoresis with immobilised pH gradients was carried out on total cell lysates and membrane fractions of Paracoccus denitrificans with the aim to characterise differences in protein expression during growth under aerobic and various anaerobic conditions (with nitrate, nitrite or nitrous oxide). Comparative image analysis of the protein pattern revealed several subgroups of the total 800 protein spots resolved that were characteristically induced or repressed in response to individual electron acceptors. The respiratory inhibitor azide also exerted a profound influence upon cellular protein composition. However, since most of the proteins showing an altered expression pattern in cells growing on oxygen differed from those in cells growing on nitrite, we suppose that azide acts mainly indirectly, possibly by influencing other cellular signals. Limited information on the P. denitrificans genome has precluded the identification of more than 8 protein spots as yet. A public accessible P. denitrificans 2-DE protein database is currently built up at http://www.mpiib-berlin.mpg.de/2D-PAGE.
ER -
BOUCHAL, Pavel, Petra PŘECECHTĚLOVÁ, Zbyněk ZDRÁHAL and Igor KUČERA. Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide. \textit{Proteomics}. Weinheim: Wiley-VCH, 2004, vol.~4, No~9, p.~2662-2671. ISSN~1615-9853.
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