BOUCHAL, Pavel, Petra PŘECECHTĚLOVÁ, Zbyněk ZDRÁHAL and Igor KUČERA. Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide. Proteomics. Weinheim: Wiley-VCH, 2004, vol. 4, No 9, p. 2662-2671. ISSN 1615-9853. |
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@article{557057, author = {Bouchal, Pavel and Přecechtělová, Petra and Zdráhal, Zbyněk and Kučera, Igor}, article_location = {Weinheim}, article_number = {9}, keywords = {Paracoccus denitrificans; Protein database; Proteome; Terminal electron acceptor; Two-dimensional electrophoresis}, language = {eng}, issn = {1615-9853}, journal = {Proteomics}, title = {Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide}, url = {http://www.wiley-vch.de/contents/jc_2120/2004/pro0833_s.pdf}, volume = {4}, year = {2004} }
TY - JOUR ID - 557057 AU - Bouchal, Pavel - Přecechtělová, Petra - Zdráhal, Zbyněk - Kučera, Igor PY - 2004 TI - Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide JF - Proteomics VL - 4 IS - 9 SP - 2662-2671 EP - 2662-2671 PB - Wiley-VCH SN - 16159853 KW - Paracoccus denitrificans KW - Protein database KW - Proteome KW - Terminal electron acceptor KW - Two-dimensional electrophoresis UR - http://www.wiley-vch.de/contents/jc_2120/2004/pro0833_s.pdf N2 - Two-dimensional gel electrophoresis with immobilised pH gradients was carried out on total cell lysates and membrane fractions of Paracoccus denitrificans with the aim to characterise differences in protein expression during growth under aerobic and various anaerobic conditions (with nitrate, nitrite or nitrous oxide). Comparative image analysis of the protein pattern revealed several subgroups of the total 800 protein spots resolved that were characteristically induced or repressed in response to individual electron acceptors. The respiratory inhibitor azide also exerted a profound influence upon cellular protein composition. However, since most of the proteins showing an altered expression pattern in cells growing on oxygen differed from those in cells growing on nitrite, we suppose that azide acts mainly indirectly, possibly by influencing other cellular signals. Limited information on the P. denitrificans genome has precluded the identification of more than 8 protein spots as yet. A public accessible P. denitrificans 2-DE protein database is currently built up at http://www.mpiib-berlin.mpg.de/2D-PAGE. ER -
BOUCHAL, Pavel, Petra PŘECECHTĚLOVÁ, Zbyněk ZDRÁHAL and Igor KUČERA. Protein composition of Paracoccus denitrificans cells grown on various electron acceptors and in the presence of azide. \textit{Proteomics}. Weinheim: Wiley-VCH, 2004, vol.~4, No~9, p.~2662-2671. ISSN~1615-9853.
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